摘要
以光棘球海胆为母本，中间球海胆为父本进行杂交试验及苗种培育。试验结果表明，通过筛选同时得到自然成熟的父母本精、卵，在比正常海胆自交精子量高40倍以上的精子作用下，杂交受精率为2．5％。在适宜的水温、光照及饵料等的培养条件下，杂交海胆能够生长附着变态，变态时间与母本相近。培育出的杂交海胆外部颜色及棘色、棘长介于两亲本之间。杂交海胆经过6～7个月的中间暂养，出苗量为0．6×10^4个／m3，个体壳径3～30mm。平均壳径8．8mm。
Two species sea urchins, Strongylocentrotus nudus （ ♀） and S. internidius（ ♂ ）, were hybridized with the average fertilization success of about 2.5 %. When the eggs of S. nudus were fertilized with sperm of S. intermedius, the amount of sperm selected synchronously was used at a rate of above 40 times than that in normal fertilization. Besides, the hybrid larvae had normal growth and metamorphosis at suitable water temperature, light and feeding regimes, and the period of whole metamorphosis were almost the same as their broodstocks. The hybrids had intermediate body color and thorns between their broodstocks. Total of 0.6×10^4 individuals/ma was obtained with test diameter from 2.59 to 2.88 mm,average of 8.8 mm during 6-7 month rearing.
出处
《水产科学》
CAS
北大核心
2009年第12期783-785,共3页
Fisheries Science
基金
辽宁省海洋渔业厅重点攻关项目
