摘要
目的：实验结果证明４ＨＰＲ对Ｈｅｌａ细胞具有较强的抑制作用，本文从细胞凋亡角度研究４ＨＰＲ抑制Ｈｅｌａ细胞生长的机理。方法：采用电子显微镜、细胞ＤＮＡ琼旨糖凝胶电泳、流式细胞仪等方法，分析４ＨＰＲ诱导Ｈｅｌａ细胞凋亡的形态学、生化学及细胞生物学改变特征。结果：电子显微镜下：细胞固缩、核膜扭曲、核染色体聚集成块并靠近核膜；细胞ＤＮＡ被降解，在１．５％琼脂糖凝胶电泳上出现典型的阶梯状图谱（ＤＮＡ
Ojection:The results of prophase experiment sbowed that44HPR had stronger inhibiton to Hela cells.To study the mechanism of 4HPR imhivition to Hcal cells growth,Methods: Using electromic microscope.DNA agarose gel electrophoresis and flow cytometer etc to analyse the chage features of morphology,hiochemicalto nuclear membrane,SNAdegradated.Typical DNAladder in 1.5% agarose gel electrophoresis;Detectd by flow cytoneter lits of subdiploid muclear peakappeared.diploid cells pesk reduced. Conclnsion:Abice results showed that 4HPR may induce Hela cells poptosis One of mechanisms of 4HPR mayinduce Hela cells spoptosis ONE of mechanisms of 4HPR inhibit to Hela cells growth was to induce apoptosis
基金
吉林科委计划项目
