摘要
目的探讨塞来昔布与顺铂联用对抑制人类子宫颈癌HeLa细胞的增效作用及其可能机制。方法用四唑盐比色法（MTT）观察塞来昔布与顺铂单独和联用对HeLa细胞的生长抑制情况，流式细胞术（FCM）检测单用塞来昔布、顺铂以及二者联合使用时HeLa细胞的凋亡率和细胞周期变化。结果塞来昔布对子宫颈癌HeLa细胞的抑制作用呈浓度和时间依赖性。塞来昔布与顺铂联合应用后对子宫颈癌HeLa细胞的抑制作用较单独用药显著增强（P=0．000），两者有协同或相加作用。塞来昔布（50μmol／L）及顺铂（16μmol／L）均可有效诱导HeLa细胞凋亡，联用凋亡率明显增加，细胞周期发生变化，表现为G0／G1期增加，S期减少。结论塞来昔布与顺铂联用对HeLa细胞有协同抗肿瘤效应。
Objective To investigate the enhancing effect of Celecoxib on the anti-proliferative effect of cisplatin （DDP） against human cervical carcinoma HeLa cells and to explore the possible mechanism. Methods After treatment of HeLa cells with Celecoxib or/and DDP, the growth suppression of HeLa cells was measured by MTT assay. Cell cycle and apoptosis were examined by flow cytometry. Results The inhibition of Celecoxib on HeLa cells was dose-dependent and time-dependent.The inhibitory effect was remarkable enhanced in asynergistic or additive pattern after treatment with the combination of Celecoxib and DDP （P =0.000） . Celecoxib （50 μmol/L） and DDP （16μmol/L） could respectively induce the apoptosis of HeLa cells and this effect of apoptosis induction was increased when combinated Celecoxib with DDP. They could change cell cycle of HeLa cells lines with G0/G1 phase cells increasing and S phase cells decreasing. Conclusion Celecoxib combined with DDP shows synergistic anti-tumor effects.
出处
《肿瘤研究与临床》
CAS
2009年第12期803-805,809,共4页
Cancer Research and Clinic
关键词
子宫颈肿瘤
药物疗法
联合
HELA细胞
塞来昔布
顺铂
Uterine cervical neoplasms
Drug therapy, combination
HeLa cells
Celecoxib
Cisplatin
