摘要
采用化学比色法,研究了银杏细胞提取物在体外对人LDL的氧化抑制和对DPPH自由基清除的作用。对LDL氧化反应潜伏期大小依次为:EGBC>BHT>>Control,EGBC、BHT对DPPH自由基的氧化抑制率分别为53.8%和42.7%。体内实验表明:EGBC可降低肝中MDA含量,提高肝中SOD和GSH-Px的活力。肿瘤对照组小鼠的胸腺明显缩小,胸腺平均湿重、红细胞Hb平均含量、红细胞及胸腺与肝中SOD活性均明显低于正常对照组。而EGBC组小鼠胸腺平均湿重、红细胞Hb平均含量、红细胞及胸腺与肝中SOD活性明显高于肿瘤对照组,Hb的含量接近正常对照组,SOD活性比正常对照组要高。结论:在实验条件下,EGBC对人LDL的氧化具有抑制作用,对DPPH自由基具有一定的清除作用,对乙醇诱导的肝损伤具有保护作用,对荷S180小鼠的肿瘤生长有抑制作用。
The inhibition effect of Ginkgo biloba cell extracts （EGBC）on human LDL oxide and DPPH radical scavenging effect of EGBC were studied in vitro with chemical colorimetric methods. The order of incubation period of LDL oxidative reaction is： EGBC〉BHT〉 〉Control The inhibition rates of EGBC and BHT on DPPH oxidation were 53.8% and 42.7%, respectively. The experiments in vivo show that EGBC can reduce the content of MDA, and improve activities of SOD and GSH-Px. The mean wet weight of thymus gland, the content oferythrocyte Hb, the activites of SOD oftumor control group were significantly lower than the normal control group, and these indexes of EGBC group were significantly higher than these of tumor control group. Besides, the Hb content of EGBC group was close to that of the normal control group, while the SOD activity of EGBC group was higher than that of the normal control group. It was concluded that EGBC can inhibit human LDL oxidation, inhibit the growth of tumor S 180, and protect liver from injury induced by ethanol.
出处
《现代食品科技》
EI
CAS
2009年第12期1396-1399,1403,共5页
Modern Food Science and Technology
