摘要
目的探讨过敏性紫癜(HSP)与CD14基因启动子多态性间的关系。方法采用病例对照研究,选择144例HSP患儿(病例组)及180例健康体检儿童(健康对照组),应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)进行CD14-159C/T和CD14-260C/T位点多态性检测,比较CD14-159C/T和CD14-260C/T位点的不同基因型与HSP患病风险的关系。应用SPSS13.0软件进行统计学分析。结果CD14-159C/T位点基因型在HSP胃肠道组、肾损害组与健康对照组中的分布存在显著性差异(P=0.041,0.010);CD14-260C/T位点基因型在单纯皮损组、肾损害组与健康对照组中的分布存在显著性差异(P=0.003,0.037),等位基因C、T在单纯皮损组、关节损害组、胃肠道组及肾损害组与健康对照组中的分布也具有显著性差异(P=0.017,0.035,0.024,0.007),且等位基因频率的相对风险分析发现,T等位基因携带者患各型HSP的风险高于C等位基因携带者(单纯皮损型:OR=2.097,95%CI:1.131～3.823;关节型:OR=1.603,95%CI:1.031～2.493;胃肠道型:OR=1.602,95%CI:1.062～2.415;肾型:OR=1.843,95%CI:1.175～2.889)。结论CD14基因启动子多态性与HSP具有内在联系,其中CD14-260C/T位点的T等位基因可能是HSP发病的遗传易感基因。
Objective To study the allele and genotype distribution of CD14 gene promoter region - 159C/T, -260C/T polymorphisms in Chinese patients with Henoch -Scho）flein purpura（HSP） , and to discuss the association between CD14 gene promoter region polymorphisms and HSP. Methods Under the case - control study, CD14 - 159C/T and CD14 - 260C/T site polymorphisms in 144 children with HSP and 180 healthy controls were analyzed with polymerase chain reaction- restriction fragment length polymorphism （PCR -RFLP）, and the relationship between CD14 - 159C/T and CD14 -260C/T site polymorphisms and the risk of HSP were analyzed. SPSS 13.0 software was used to analyze the data. Results The distribution of CD14 gene - 159C/T polymorphism was significantly different between gastro - intestinal（GI） involvement group, renal involvement group and healthy control group（ P = 0. 041,0. 010, respectively） ;but the CD14 gene -260C/T polymorphism was significantly different between simple lesion group, renal involvement group and healthy control group（ P = 0. 003,0. 037, respectively） and C and T allele were significantly different between simple lesion group, joint damage group, GI involvement group,renal involvemerit group,healthy control group （P = 0. 017,0. 035,0. 024,0. 007 ）and the relative risk for different types of HSP in T allele carriers was higher than that in C allele carriers （ simple lesion ： OR = 2. 097,95 % CI 1.131 - 3. 823 ;joint ： OR = 1. 603,95 % CI 1.031 - 2. 493 ; GI ： OR = 1. 602,95% CI 1.062-2. 415 ;renal：OR = 1. 843,95% CI 1. 175 -2. 889 ;respectively）. Conclusions CD14 gene promoter region polymorphism is associated with HSP and T alele of CD14 - 260C/T may be a risk factor for HSP.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2009年第21期1638-1640,共3页
Journal of Applied Clinical Pediatrics
基金
安徽省科技攻关项目资助(07010300198)
关键词
过敏性紫癜
CD14
基因多态性
儿童
Henoch - Schoenlein purpura
CD,4
gene polymorphism
child
