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MTH2蛋白与8-氧鸟嘌呤脱氧核苷在阿尔茨海默病患者海马组织中的表达变化 被引量：1

The increase of 8-oxo-2'-deoxyguanosine and the decrease of MutT homolog 2 in the hippocampi of patients with Alzheimer's disease
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摘要 目的:观察8-氧鸟嘌呤脱氧核苷三磷酸酶同源蛋白2(MTH2)及8-氧鸟嘌呤脱氧核苷(8-oxodG)在阿尔茨海默病(AD)患者海马组织标本中的表达变化,探讨在AD患者海马中二者表达的相关性。方法:选用临床及病理均诊断为AD的尸检海马组织石蜡切片作为实验组,年龄匹配且临床及病理诊断均排除AD的尸检海马组织石蜡切片作为对照组,每组各3例,用免疫组化的方法分别检测人脑海马中8-oxo-dG的含量及MTH2蛋白的表达。结果:免疫组化定量结果显示,在人脑海马组织CA1及CA3区,AD患者8-oxo-dG的表达显著高于年龄匹配对照组,而AD患者MTH2的表达显著低于年龄匹配的对照组。结论:在人脑海马组织中,DNA氧化产物8-oxo-dG含量在AD患者比年龄匹配对照组有显著提高,抗氧化修复蛋白MTH2的表达量却显著降低,提示人脑海马组织中MTH2蛋白表达量的减少及8-oxo-dG含量的增加与AD的发病过程相关。 Objective：To investigate the changes of 8-oxo-2＇-deoxyguanosine （8-oxo-dG）amount and the expression of Mutt homolog 2（MTH2）in the hippocampi of Alzheimer＇s disease（AD）patients.Methods：The＇aherations of 8-oxodG and MTH2 expression were analysed in hippocampi from autopsy specimen from three AD patients and three age-matched controls by means of immunohistochemistry using specific antibodies.Results： Semiquantitative immunohistochemical results indicated,in the CA1 and CA3 regions of hippocampus of AD patients,the contents of 8-oxo-dG were significantly higher than those in control subjects,and the expressions of MTH2 were significantly lower than those in control subjects.Conclusion：The increase of 8-oxo-dG and the decrease of the expression of MTH2 presented in a synchronizing style was observed in the hippocampus of AD patients.The result suggested that the ongoing accumulation of 8-oxo-dG and the difficiency of nucleotide repair enzyme MTH2 might be relevant with the pathogenesis of AD.
作者 宋晓宁 郑君德 刘东戈 黑爱莲 蔡剑平
机构地区 北京医院 广州医学院第一附属医院检验科 北京医院病理科
出处 《中日友好医院学报》 2009年第5期287-290,F0002,共5页 Journal of China-Japan Friendship Hospital
基金 科技部国际科技合作计划(2006DFB31410)
关键词 8-氧鸟嘌呤脱氧核苷 MTH2蛋白2 阿尔茨海默病 8-oxo-2＇-deoxyguanosine MutT homolog 2 Alzheimer＇s disease
分类号 R749.16 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献12

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二级参考文献47

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共引文献25

同被引文献15

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中日友好医院学报
2009年 第5期

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