摘要
不同的温度、福尔马林浓度、灭活的时间以及灭活菌液的浓度均可影响中华鳖嗜水气单胞菌的灭活。本研究得到了菌液浓度为６０×１０８ｃｆｕ／ｍｌ时的９种灭活方法。通过琼脂双扩散试验对灭活菌液抗原性比较，认为２８℃、０．２％的福尔马林灭活２ｄ为鳖嗜水气单胞菌较理想的灭活方法。
The inactivation of Aeromonas hydrophila of softshelled turtles, Trionyx sinensis , was influenced by different temperatures, concentrations of formalin, inactivating lengths and numbers of bacteria in 0.8% of PS. Nine inactivating methods were obtained when the concentration of bacteria was 60×10 8 cfuml. After the antigenicities of those bacterins were compared by double diffusion test, the inactivating method to strain T3 with 0.2% of formalin at 28 ℃ for 2 d was the optimal one of all.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1998年第4期389-394,共6页
Journal of Huazhong Agricultural University
基金
国家自然科学基金
农业部"九五"重点项目
关键词
中华鳖
嗜水气单胞菌
灭活
Trionxy sinensis , Aeromonas hydrophila , inactivation
