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整合素αvβ3受体靶向的MR分子探针制备及体外成像 被引量：7

Preparation of MR molecular probe targeting integrin αvβ3 receptor and its in vitro imaging
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摘要 目的:构建结缔组织生长因子多肽(CTGFP)超微超顺磁氧化铁粒子(USPIOs)磁共振(MR)分子探针,以显示高表达整合素αvβ3受体人肝癌Bel7402细胞。方法:用免疫细胞化学方法检测Bel7402细胞αvβ3受体表达情况;用邻苯二马来酰亚胺(OPDM)将USPIO和CTGFP偶联;将USPIO-CTGFP、USPIO分别与Bel7402细胞孵育,同时设立多肽竞争组及对照组,行普鲁士蓝染色显示铁颗粒,体外磁共振成像(MR I)观察在T2*W I上T2弛豫时间变化。结果:Bel7402细胞αvβ3受体表达阳性;普鲁士蓝染色可见USPIO-CTGFP组铁颗粒分布在细胞膜,而USPIO组及竞争组细胞膜上和细胞内铁颗粒极少;体外MR I显示USPIO-CTGFP组T2弛豫时间低于USPIO组、竞争组及对照组(P<0.05)。结论:USPIO-CTGFP对αvβ3受体具有较好靶向作用,可通过MR I显示高表达整合素αvβ3受体Bel7402细胞。 Objective To synthesize a novel MR molecular probe of ultra superparamagnetic iron oxide particles （USPIOs） containing connective tissue growth factor peptide （CTGFP） and to display integrin αvβ3 receptor highly expressed on human hepatic carcinoma cells （BeL7402 cells）. Methods The expression of integrin αvβ3 receptor on BeL7402 ceils was detected by immuocytochemistry. The CTGFP and USPIOs were conjugated by N, N＇ - O- phenylenedi- maleimide （OPDM）. The BeL7402 cells were incubated with USPIO-CTGFP and USPIOs respectively. Meanwhile, the competition experiments and the control experiments were performed. Prussian blue staining was conducted to show iron. Magnetic resonance imaging （MRI） was performed to observe the T2 relaxation time by using T2 ＊ WI sequence. Results The results of immuocytochemistry showed that integrin αvβ3 receptor was positively expressed on BeL7402 cells. The prussian blue staining revealed that there was large amounts of iron binding with integrin αvβ3 receptor in BeL7402 cells, while there was little iron in the USPIO group and the competion group. T2 relaxation time of cells treated with US- PIO- CTGFP was shorter （ P 〈 0. 05） than that of others. Conclusion The MR molecular probe, USPIO- CTGFP,can effectively target integrin αvβ3 receptor and can be applied to display integrin αvβ3 receptor expressed on BeL7402 cells.
作者 王小东 吴国球 居胜红 滕皋军 姚玉宇 刘乃丰
机构地区 东南大学附属中大医院心内科 东南大学附属中大医院检验科 东南大学附属中大医院放射科
出处 《东南大学学报（医学版）》 CAS 2009年第6期470-473,共4页 Journal of Southeast University(Medical Science Edition)
基金 国家自然科学基金资助项目(30840042 30970809 30830039 906060007)
关键词 结缔组织生长因子 多肽 分子影像学 分子探针 超微超顺磁氧化铁粒子 connective tissue growth factor peptide molecular imaging molecular probes ultra superparamag netic iron oxide particles
分类号 R445.2 [医药卫生—影像医学与核医学]
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参考文献11

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同被引文献73

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二级引证文献26

东南大学学报（医学版）
2009年 第6期

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