摘要
目的：建立气相色谱测定血清中碘的方法。方法：不同的衍生条件中,血清中碘和丁酮衍生成碘丁酮,再经HP-5（30 m×0.25 mm×0.25μm）毛细管柱分离,气相色谱电子捕获检测器检测。结果：在酸性环境、亚硫酸钠及3.5%双氧水衍生条件下,97%的碘酸盐中的碘转化为单质碘,与丁酮衍生成碘丁酮;该方法用于血清中碘的分析,测得血清中碘的浓度为780μg/L,碘的检出限为4μg/L,线性范围4～100μg/L,相关系数r=0.9992,加标回收率83.0%～95.0%,RSD为3.8%。结论：此方法能够用于血清中总碘含量的检测。
Objective：To establish a method for determination of iodine in serum by gas chromatography.Methods：In different derivative matrix,iodine in serum was derived by butanone and the derivative was extracted by n-hexane.The derivative was separated by HP-5 capillary column（30 m×0.25 mm×0.25 μm） and detected by gas chromatography-electon capture detector.Results：In derivative matrix including acidification,sodium sulfite and 3.5% hydrogen peroxide,the iodate was derived by butanone,the derivative efficiency was 97%.The method was applied to analyze iodine,iodine in serum was 780 μg/L,the linear range was 4～100 μg/L.The correlation cofficiency was 0.9992,the recovery 83.0%～95.0%,RSD 3.8%.Conclusion：The method is suitable to analyze the iodine including iodate and iodide in serum.
出处
《中国卫生检验杂志》
CAS
2009年第12期2805-2806,共2页
Chinese Journal of Health Laboratory Technology
关键词
碘
血清
气相色谱
Iodine
Serum
Gas chromatography
