摘要
目的:通过对同一检验科不同PCR检测系统进行比较和偏倚评估,探讨不同PCR检测系统间解脲支原体(U-U)及沙眼衣原体(CT)的测定结果是否具有可比性,为DNA测定的标准化和实验室认可提供数据。方法:用达安公司DA7600以及美国罗氏公司的Light Cycler荧光定量PCR仪对患者样品同时进行检测。结果:不同检测系统对同一患者样品进行UU和CT的检测结果差异无统计学意义(P>0.05)。结论:当同一实验室同一检验项目存在2个或2个以上的检测系统时,应进行比较和偏倚评估,判断其临床可接受性,以保证检验结果的可比性。
Objective：Through the same test subjects of different PCR detection system comparison and bias evaluation,to explore the comparison of the results using different PCR detection system between Ureaplasma urealyticum （UU） and Chlamydia trachomatis （CT）,and to provide the standardization of DNA and clinical laboratory authorized the experimental data. Methods：The patients＇ specimen were detected using two different detection systems which were DA7600 from da＇an company and the Light Cycler from ROCHE company. Results：The deviation of the two different detection systems was acceptable,there was no significant difference （P0.05）. Conclusion：When the number of the same test items are more than 2 or 2 detection systems in the same laboratory,the test results should be carried out to compare and impartial assessment to determine its clinical acceptability in order to ensure the comparability of test results.
出处
《中国医药导报》
CAS
2009年第36期83-84,共2页
China Medical Herald
