摘要
目的:建立一种快速而准确的检测血清成骨生长肽浓度的方法。方法:制备具有较高效价的成骨生长肽抗体,通过竞争性酶联免疫吸附法及其改进方法建立检测成骨生长肽浓度的标准曲线。结果:亲和纯化的抗体比盐析抗体的标准曲线更好,改进的竞争性酶联免疫吸附法方法比普通的竞争性酶联免疫吸附法方法的检测灵敏度高一个数量级,可满足临床检测血清成骨生长肽浓度灵敏度的要求。结论:改进的竞争性酶联免疫吸附法可以为临床研究提供可靠而灵敏地检测血清成骨生长肽含量的方法。
Objective： To develop an enzyme immunoassay for the detection of Osteogenic Growth Peptide in serum for clinical investigation.Methods： Two kinds of competitive Enzyme linked immununosorbent assay were employed,based on sensitivity and shape of dose-reaction curve, the most suitable testing pattern was selected for further application. Results： Purified antibody was better than salting-out antibody in competitive Enzyme linked immununosorbent assay in terms of standard curve. Modified competitive Enzyme linked immununosorbent assay was better than competitive Enzyme linked immununosorbent assay in sensitivity. Conclusion： Modified competitive Enzyme linked immununosorbent assay for Osteogenic Growth Peptide is sensitive enough for clinical investigation.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2009年第12期1116-1118,共3页
Chinese Journal of Immunology
基金
山东省自然科学基金项目资助(No.Y2007C153)
关键词
成骨生长肽
竞争性酶联免疫吸附法
骨质疏松
骨折愈合
Osteogenic Growth Peptide
Enzyme linked immununosorbent assay
Osteoporosis
Fracture healing
