摘要
目的研究细胞外间质对牛角膜内皮细胞增殖的影响。方法以８０４Ｇ细胞外间质作为牛角膜内皮细胞培养的基础，用免疫荧光分析结合素α６的表达，并测定酸性磷酸酶活性分析牛角膜内皮细胞增殖。结果生长于８０４Ｇ细胞外间质上的牛角膜内皮细胞结合素α６表达增加，酸性磷酸酶活性明显高于对照组。
AIM To study the effect of extracellular matrix on bovine corneal endothelial cells. METHODS The integrin α6 expression of bovine corneal endothelial cells cultured on 804G extracellular matrix was observed by immuno fluorescence and acidic phophatase was measured. RESULTS The integrin α6 of bovine corneal endothelial cells cultured on 804G extracellular matrix was overexpression and activity of acidic phosphatase was significantly higher than control. CONCLUSION 804G extracellular matrix can lead to proliferation of bovine corneal endothelial cells in vitro.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1998年第4期348-350,共3页
Chinese Pharmacological Bulletin
关键词
细胞外间质
角膜
内皮细胞
细胞增殖
extracellular matrix
cornea
endothelium
integrin α6
proliferation
expression
bovine
