摘要
目的创建大鼠心搏骤停与心肺复苏模型。方法成年Sprague-Dawley（SD）大鼠30只,随机分成对照组（生理盐水组n=15）、肾上腺素组（n=15）。采用交流电生理刺激仪经食道调搏诱发心搏骤停模型,成功后5min开始心肺复苏,应用自行设计大鼠心脏胸外按压机进行心脏按压。观察心搏骤停类型分布、诱发成功时间、自主循环恢复率及复苏成功率。处死大鼠解剖观察心脏有无灼伤,内脏有无破裂。结果所有大鼠均诱发心搏骤停成功,其中心室颤动10例（33.3%）、无脉搏电活动15例（50.0%）、心电静止5例（16.7%）、诱发时间60-150s;自主循环恢复率对照组为1例（6.7%）、肾上腺素组为7例（46.6%）,复苏成功率对照组为0、肾上腺素组为33.3%。食道黏膜轻度灼伤,心脏未见灼伤,食道及腹腔内脏未见破裂。结论经食道交流电调搏可建立良好的大鼠心博骤停模型,应用大鼠电动心脏复苏器可达到标准化复苏实验要求。
Objective To establish a cardiac arrest and cardiopulmonary resuscitation model. Methods Thirty Sprague-Dawley（SD） rats were randomly divided into control group（n=15） and epinephrine group（n=15）. A rat model of cardiac arrest was established with an alternate current （AC） electrode placed in esophagus. Five minutes after the onset of cardiac arrest, cardio-pulmonary resuscitation （CPR） was initiated. Cardiac compression was performed with a machine made by our group. Distribution of cardiac arrest types, pace-inducing time, recovery of self circulation （ROSC） and success resuscitation were observed. The rats were killed to examine whether there was any burn of the esophagus mucosa and heart, and rupture of the abdominal organs. Results Cardiac arrest was successfully induced in all rats, including ventricular fibrillation （VF） in10 rats （33.3%）, pulseless electric activity （PEA） in 15 rats （50%）, asystole in 5 rats （16.7%）, respectively. The pacing time was 60-150s. The ROSC was achieved in 1 rat of the control group （6.7%） and in 7 rats of the epinephrine group （46.6%）, respectively. The success resuscitation was achieved no rat of the control group （0%） and in 5 rats of the epinephrine group （33.3%）, respectively. Mild burn was observed in esophagus mucosa while no heart burn and rupture of esophagus and abdominal organs were found. Conclusion A rat cardiac arrest model can be established with an AC electrode placed in esophagus. Cardiac compression with a machine can meet the requirements of a standard CPR study in rats.
出处
《军医进修学院学报》
CAS
2009年第6期875-877,共3页
Academic Journal of Pla Postgraduate Medical School
基金
解放军总医院苗圃基金(07MP074)
关键词
心脏停搏
心肺复苏术
大鼠
模型
heart arrest
cardiopulmonary resuscitation
rats
models
animal
