摘要
采用了多种免疫方式及一种新的检测方法：用经固定的杂交瘤细胞（Ａｂ１）包被酶标板，加入待测上清孵育后加入只与哺乳类动物抗体Ｆｃ段结合的ＨＲＰＰｒｏｔｅｉｎＡ。共得到了６株分泌抗独特型单抗的杂交瘤细胞。其中两株与Ａｂ１的结合可被腺病毒抑制，且都能在Ｂａｌｂ／ｃ小鼠诱导出与腺病毒结合的Ａｂ３。因此为Ａｂ２β。
A new detecting test has been developed to raise monoclonal antiidiotypic antibodies.The results presented here demonstrate that direct fixation tests in which the hydridoma cells are used as idiotype bearing carriers and HRPProtein A applied may also be used routinely. 6 lines of hybridoma secreting monoclonal antiidiotypic antibodies were obtained.Binding of Ab2 52 and 41 to Ab1 342 could be inhibited by adenovirus.Both of 52 and 41 could induce Ab3 binding to antigen(adenovirus).They are Ab2β.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第5期378-379,共2页
Chinese Journal of Immunology
