摘要
目的研究特发性血小板减少性紫癜(ITP)患者外周血CD4+CD25+调节性T细胞数量及Foxp3基因的表达水平,探讨它们在ITP发病机制中的作用。方法分别收集30例ITP患者及健康对照组外周抗凝静脉血,分离纯化T淋巴细胞。PE标记的抗CD4单抗,FITC标记的抗CD25单抗,作双色流式细胞术,分析ITP患儿外周血CD4+CD25+调节性T细胞百分率,RT-PCR检测T细胞Foxp3 mRNA表达水平。结果ITP者外周血CD4+T、CD4+CD25+T细胞百分率及T细胞Foxp3 mRNA水平均低于对照组(P﹤0.01),并且CD4+CD25+T细胞百分率与Foxp3mRNA水平呈正相关。结论ITP患者外周血存在细胞免疫功能失调,CD4+CD25+调节性T细胞数量与功能状态发生改变,T淋巴细胞耐受机制的破坏可能与ITP的发病机制有关。
Objective To investigate the quantity of peripheral blood CD4^＋CD25^＋ regulatory T cell, the expression of Foxp3 gene and the pathogenesis of idiopathic thrombocytopenic purpura （ITP）. Methods Anti-coagulated venous blood by heparin was collected from ITP children and healthy people. T cells were collected by human CD3^＋T cell enrichment column. The expression of Foxp3 mRNA on T cell was analysed by RT-PCR. The quantity of peripheral blood CD4^＋CD25^＋ regulatory T cell was detected by immunofluoreseenee staining and bicolor flow eytometry by CD25/CD4 gating. Results The expression of CD4^＋CD25^＋T cell and the level of Foxp3 mRNA in ITP were significantly lower than those of healthy people （P〈0.05）. there were positive correlation between them（P〈0.05）. Conclusions There is peripheral blood eelluar immunological function disorder in ITP patient, and the abnormal expressions of CD4^＋CD25^＋T cell and Foxp3 mRNA on T cell may involve in ITP immunopathogenesis.
出处
《实验与检验医学》
CAS
2009年第6期589-590,594,共3页
Experimental and Laboratory Medicine
