摘要
目的应用二维电泳(2-DE)技术研究急性弓形虫感染对小鼠脑蛋白质组的改变。方法分别提取纯化急性弓形虫感染和正常同窝配对的C57BL/6J小鼠脑组织蛋白,以固相pH3～10梯度聚焦为第一向,SDS-PAGE垂直电泳为第二向进行双向凝胶电泳,应用PDQuest 1.0软件分析蛋白差异。结果急性弓形虫感染与正常同窝配对的C57BL/6J小鼠脑组织2-DE图谱分别检测出(132±10)个和(170±13)个蛋白斑点,对两组电泳图谱进行匹配后,有19个蛋白点仅在急性弓形虫感染小鼠脑蛋白2-DE图谱中表达;另有37个蛋白斑点仅在正常同窝配对小鼠中表达。部分蛋白在两组小鼠脑组织中含量也发生明显变化。结论急性弓形虫感染小鼠脑蛋白质有明显改变,为研究弓形虫感染致脑组织损害及筛选治疗新药提供了有益的线索。
Objective To explore the impact of acute Toxoplasma gondii infection on cerebral proteins and nerve growth in mice by 2D electrophoresis.Methods The cerebral proteins from C57BL/6J mice infected with Toxoplasma gondii and normal paired mice were extracted.The discrepant proteins were checked by 2D electrophoresis.Isoelectric focusing was determined as the first direction（immobilized pH gradient gel 3-10）,SDS-PAGE as the second direction to execute 2D electrophoresis,and PDQuest 1.0 software was used to analyze 2D electrophoretogram.Results The protein spots in Toxoplasma gondii infected mice and normal paired mice were（132±10） and（170±13）,respectively.After the analysis by PDQuest 1.0 software,only 19 protein spots were found to express in infected mice and only 37 protein spots were found to express in normal paired mice.Additionally,the obvious quantitative changes in a part of proteins of the cerebrum in the both group occurred.Conclusion There are obvious changes in cerebral proteins from mice with acute Toxoplasma gondii infection,which provids useful clues for studying the cerebral proteins injury in acute Toxoplasma gondii infected mice and the new cure drug.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2009年第6期507-510,共4页
Chinese Journal of Schistosomiasis Control
基金
江苏省卫生厅基金(X200739)
关键词
弓形虫感染
小鼠
脑
蛋白质组学
二维电泳
Toxoplasma gondii infection
mice
Cerebrum
Proteomics
Two-dimensional electrophoresis
