摘要
目的建立枇杷叶中齐墩果酸和熊果酸的含量测定方法。方法采用毛细管区带电泳,高频电导检测;未涂层弹性融硅石英毛细管柱55cm×75μmID,有效长度46cm;1.2mmol·L-1三乙胺-HCl（pH=10.60）,0.24mmol·L-1β-环糊精为运行缓冲液;分离电压12.5kV;重力进样10s（高度20cm）。结果以布洛芬为内标,齐墩果酸、熊果酸线性范围分别为5.1～102.0μg/ml（r=0.9997）和5.55～111.0μg/ml（r=0.9993）;平均回收率分别为96.0%和97.2%。结论该法简便、准确、快速、重现性好,可用于枇杷叶的质量控制研究。
objective To establish a method for the determination of oleanolic acid and ursolic acid isomers in Folium eriobotryae. methods It was studied by Capillary Zone Electrophoresis with high frequency conductivity detection. The separation was performed on a uncoated fused silica capillary of 55 cm×75 μm ID（46 cm effective length）. 1.2 mmol·L-1 Triethylamine（TEA） buffer containing 0.24 mmol·L-1 β-cyclodextrin and pH adjusted to10.60 with Hydrochloric acid was selected for the running buffer. The voltage applied was 12.5 kV and temperature was maintained at 25℃. The sample was injected by gravity（10 s,20 cm）. results The linear ranges of determination for oleanolic acid and ursolic acid were 5.1-102.0 μg/ml（r=0.9997）和 5.55-111.0 μg/ml（r=0.9993）. The average recovery for oleanolic acid and ursolic acid were 96.0%、97.2%. conclusion It was proved that the method was reliable,simple,rapid and accurate. The method can be used for quantitative determination of oleanolic acid and ursolic acid isomers in Folium eriobotryae.
出处
《中国中医药现代远程教育》
2009年第12期60-62,共3页
Chinese Medicine Modern Distance Education of China
基金
广东省自然科学基金资助项目(№.5002841)
关键词
毛细管电泳
高频电导检测
枇杷叶
齐墩果酸
熊果酸
Capillary Zone Electrophoresis
High Frequency ConducTivity Detection
Folium eriobotryae
Oleanolic acid
Ursolic acid
