摘要
在获得转fad2基因ihpRNA的甘蓝型油菜的基础上,应用气相色谱技术分析了转基因株系W-4(T_1代)单株自交种子(T_2代)的脂肪酸组成。结果显示:由49个单株组成的T_2代群体油酸(C_(18:1))含量变幅为55.87%～84.73%,其中有19个(占38.8%)单株种子油酸含量大于75%,有11个(占22.4%)单株种子油酸含量大于80%;受体(对照)的油酸含量变幅为55.28%～67.43%;获得的转基因高油酸材料(油酸含量≥75%)种子中亚油酸(C_(18:3))和亚麻酸(C_(18:3))含量明显低于受体;高油酸材料的油酸脱饱和指数ODP值为5%～12%,而受体的ODP值高达20%～36%。基于ODP值,可以推测转基因高油酸材料种子中的FAD2活性受到有效抑制,从而阻止了油酸向亚油酸、亚麻酸的合成。
The seffed seeds of 49 T1 individuals from the transgenic line W-4, transformed by the construct containing ihpRNA targeted to the endogenous fad2 gene of Brassica napus L. , were harvested and the seed fatty acids were analyzed by gas ehromatogram. The results showed that the variation range of oleic acid level among the T2 seeds of 49 progenies was 55.87% to 84. 73% much wider than that of the control, and that of the control was about 55.28% to 67.43%. There were 19 individuals, accounting for 38.8% of the total plants, that contained greater than 75% oleic acid in the seed oil, including 11 individuals with more than 80% oleic acid, which accounted for 22.4% of the total plants. These high oleic acid lines were also found considerable reductions in linoleic acid and linolenic acid. The oleic acid parameter （ODP） of the high oleic acid lines was only 5% -12% much lower than that of the control, which had an ODP value of around 20% -36%. Based on the ODP value, it could be assumed that about 60%-83% FAD2 activity in the seeds of high oleic acid lines was effectively inhibited, probably owing to the expression of ihpRNA for fad2 gene in the seeds of the transgenic plants, thereby preventing the oleic acid to be desaturated to linoleic acid and linolenic acid further.
出处
《江苏农业学报》
CSCD
北大核心
2009年第6期1234-1237,共4页
Jiangsu Journal of Agricultural Sciences
基金
国家"863"项目(2006AA100106)
农业部"948"项目(Q04)
江苏省自然科学基金项目(BK2006161)
关键词
高油酸种质
转基因油菜
△-12脱饱和酶基因
high oleic acid germplast
transgenic Brassica napus L.
△-12 desaturase gene
