摘要
为建立有效的不育基因分子标记辅助育种平台,以5个核不育两用系为研究材料,选用20条随机引物,利用RAPD混合集群分离分析法,对各两用系可育与不育基因池进行分子标记的初步分析。结果表明,在油研10号筛选到2个与不育基因相关的标记:BA2084-600和S1354-1300;在萝×诸中筛选到1个与不育基因相关的标记:LC02-500。在827AB、T18筛选到集团间有差异的引物,未筛选到通过集团单株检测的与其育性基因相关的标记。通过RAPD混合BSA法可以筛选到与不育基因相关的特异DNA标记。
The molecular markers of fertile and sterile gene pool of 5 dual purpose lines with nucleic male sterile gene were analyzed by using 20 random primers to establish the supplemental breeding platform of effective sterile gene molecular markers. The results showed that two markers of BA2084-600and S1354-1300 related to sterile genes were selected from Youyan 10, the marker of LC02-500 related to sterile genes was selected from Raphnua sativus ×Orychophragmus violaceus, and the different primers were selected 13tom 827AB and T18 but the markers related to fertile genes were not selected from 827AB and T18. The specific DNA markers related to sterile genes could be selected by using the combination method of RAPD and BSA.
出处
《西南农业学报》
CSCD
北大核心
2009年第6期1522-1525,共4页
Southwest China Journal of Agricultural Sciences
基金
贵州省科技攻关项目"油菜分子标记辅助育种技术研究"[黔科合NY字(2006)3007]
贵州省科技攻关项目"甘蓝型油菜含油量基因的QTL分析"[黔科合NY字(2007)3006]
关键词
核不育两用系
不育基因
分子标记
RAPD
Dual purpose line with nucleic male sterile line
Sterile gene
Molecular marker
RAPD
