摘要
应用目前HLA研究领域中成熟的,有效的PCR-RFLP基因分型技术,从DNA水平对藏族健康群体进行了HLA-DQA1(49人)和-DQB1(49人)基因分型,这在国内外属首次。所采用的PCR-RFLP基因分型技术是在HLA-DQA1和-DQB1各等位基因全部序列已知的情况下,对其第2个外显子碱基序列扩增进而进行RFLP分析的方法。这种方法得到的RFLP的所有片段都是已知序列,因而精确度很高,同时为发现新的等位基因提供了成熟而有效的分析方法。研究结果表明,在藏族DQA1的8个等位基因,DQA1*0301的基因频率最高(36.74％)。DQA1*0601(4.08％)、*0103(4.08％)和*0401(5.10％)最低。在DQB1的16个等位基因中,OQB1*0302(16.33％)、*0303(15.31％)和*0602(15.31％)为最常见,没有观察到*0504。统计分析表明,在DQA1各等位基因分布上,藏族与新疆汉族、北方汉族、上海汉族十分相近;与维吾尔族和哈萨克族也没有明显差异。在OQB1各等位基因的分布上,藏族与汉族、维族、哈族之间略有差异,而汉族、维族、哈族之间也存在一些差异。
HLA-DQA 1 and -DQB1 98 chromosomes alleles were investigated in the Tibetan of Qinghai Province using PCR-RFLP genotyping method. Of the 8 alleles in DQA1 detected, DQA1 * 0301 is the most common allele in the Tibetan, and * 0601, * 0103, * 0401 are the rarest. There are some differences between the Tibetan and the Uygur or the Kazak. There is a significant difference between the Tibetan and the Han People. Of the 16 alleles in DQB1 detected, DQB1 * 0302, * 0303 and * 0602 are the most common alleles, and * 0504 is absent. In DQB1 alleles, the gene frequencies of the Tibetan are different from the Uygurs, the Kazaks and the Han People
基金
国家自然科学基金资助课题(No.39370390)
