摘要
以深黄被孢霉（Mortierellaisabellina）AS3．3410为出发菌株，经紫外线，硫酸二乙酯和亚硝基胍复合诱变处理，选育成功高产脂深黄被抱霉M018变株，其摇瓶培养菌体油脂含量达65．6％，比出发菌株提高133％。60m3罐三级发酵培养菌体油脂含量高达79．2％，生物量达37．8g／L．气相色谱分析表明变株M018r-亚麻酸的含量比出发菌株提高了53％。连续传代试验表明M018是一稳定的变株。该变株油脂合成的最佳碳源为葡萄糖，最佳氮源为酵母膏，最佳C／N为60：1，中温（25～30℃）适于菌丝生长，低温（15～20℃）适于细胞油脂合成。代谢曲线表明M018变株是在培养基残氮极低时，才开始将葡萄糖大量转化合成油脂。
A mutant sabin M018 accumulating a large amount of lipid was successful obtainedwhen the origind strain Mortierella isabellina AS3.3410 was treated by UV, DES, NTG asmutating agent The content or lipid in M018 mycelium cultured in 500ml shaking flask was up to65.5%, which was 133% higher than that in the original strain mycelimp and the content of findin M018 mycelium cultured in 60m3 fermentor was up to 79.2% with biomass 37.89 / L Analysisby gas chromatograph indicatod content of r-lindenic exishng in lipid from M018 mycelium was7.2% which was 53% more than that from AS3.3410 mycelium Pass-generation test showed thatthe heredity character of mutant M018 was stable. The optimal medium for M018 producing lipidwas made up of glucose as carbon source and yeast extract as nitrogen scarce with C /Nratio 60: 1.TemperatUre 25~30℃ was suitable for M018 mycelium growing and 15~20℃was more suitablefor the mycelium producing lipid The fermentation come curve shoWed that the mutant M018began to rapidly convert glucose exishng in fermentative medial into lipid when the content ofnitrogen scarce was very low. Ion Zn2+ could improVe idion value of lipid
出处
《微生物学通报》
CAS
CSCD
北大核心
1998年第4期187-191,共5页
Microbiology China
基金
国家火炬高新技术产业项目
关键词
被孢霉
选育
油脂合成
发酵
Mortierella isabellina, Breeding, Lipid, Fermentation
