摘要
目的:探讨抗环瓜氨酸肽(CCP)抗体的检测在类风湿关节炎(RA)诊断中的价值。方法:采用酶联免疫吸附法(ELISA法)、间接免疫荧光法、速率散射比浊法分别检测RA组202例、非RA组(其他风湿病患者)103例和正常对照组(正常献血人员)20例的抗CCP抗体、抗角蛋白抗体(AKA)和类风湿因子(RF)。结果:RA组抗CCP抗体的阳性率为82.67%(167/202),非RA组为3.83%(4/103),正常对照组为0%(0/20)。抗CCP抗体对RA诊断的敏感性和特异性分别为82.67%、94.17%,RF对RA诊断的敏感性和特异性分别为86.63%、71.84%,AKA对RA诊断的敏感性和特异性分别为8.91%、99.03%。在RA患者中抗CCP抗体与AKA阳性率比较差异有统计学意义(P<0.01),抗CCP抗体与RF比较差异无统计学意义(P>0.05),3种方法的检测结果间存在相关性。结论:用ELISA法检测血清中抗CCP抗体简便、结果可靠,对RA诊断具有高度的敏感性和特异性。
Objective： To detect anti-cyclic eitrullianted peptide （CCP） antibody in patients with rheumatoid arthritis （RA） and explore its clinical value. Methods： Enzyme linked immunosorbent assay （ELISA）, indirect immuneofluorescence （IIF）, rate nephelometry were used to detect anti-CCP antibodies, Anti-kera- tin antibodies （AKA） and Rheumatoid factor （RF） in patients with 202 RA, 103 non- RA other rheumatic cases and 20 normal donors group. Results： Among 202 patients with RA, 167patients showed positive for anti-CCP （82.67% ）, whereas only 3. 83% in 103 non-RA group. The sensitivity and specificity of anti- CCP antibodies were 82. 67%, 94. 17% in RA respectively. The sensitivity and specificity of RF were 86.63% , 71.84% in RA respectively. The sensitivity and specificity of AKA were 8.9%, 99.03% in RA respectively. Anti-CCP antibodies were more sensitive and specific than RF and AKA for diagnosing RA, although there were correlations among three kinds of antibodies. Conclusion： Detecting anti-CCP antibod- ies by/ELISA may be a brief, standard and reliable method. Anti-CCP antibodies can be considered as one kind of masker antibody for RA.
出处
《新疆医科大学学报》
CAS
2009年第12期1732-1734,共3页
Journal of Xinjiang Medical University
关键词
抗CCP抗体
类风湿关节炎
类风湿因子
抗角蛋白抗体
anti-cyclic citrullinated peptide antibodies
rheumatoid arthritis
anti-keration antibodies
rheumatoid factor
