摘要
目的了解不同病期支气管哮喘(简称哮喘)患者外周血中嗜酸粒细胞(EOS)上前列腺素D2(PGD2)受体(DP1/CRTH2)结合容量改变情况。方法随机病例对照研究。分为正常对照组24例,平均年龄(5.8±2.7)岁,哮喘急性发作期组18例,平均年龄(4.7±3.8)岁和缓解期组12例,平均年龄(3.9±3.1)岁。用放射配体结合分析法测定外周血EOS上DP1/CRTH2结合容量。结果哮喘急性发作期组和缓解期组患者EOS数显著增加(×106/L)(急性发作期组:297±58;缓解期组:157±34和对照组95±26)(P<0.01),EOS上CRTH2受体结合容量和总结合容量比正常对照组和缓解期组显著增加(fmol/106细胞)(CRTH2分别为:245.58±48.27、154.13±67.25、119.29±53.47;DP总结合分别为:456.14±25.57、337.51±38.52、301.22±39.54)(P<0.01)。结论哮喘急性发作期组CRTH2受体结合容量增加,PGD2激发的EOS的趋化反应将偏向于CRTH2受体中介的生物学效应。
Objective To investigate the change of prostaglandin D2 receptor （DP1/CRTH2） on eosinophil（EOS） in exacerbation and remission phase patients with bronchial asthma（asthma）. Methods Randomized case control clinical trial was designed. Thirty patients were randomly divided into two groups： exacerbation phase group 18 cases（4.7± 3.8） years, and remission phase group（3.9 ± 3.1） years, normal control was 24 cases（5.8± 2.7） years. Radiological binding assay was used to study the DP1/CRTH2 receptors on EOS in peripheral blood. Results The EOS counts in exacerbation phase group were significantly higher than those in remission phase group and normal controls（ × 10^6/L） （exacerbation phase group,297± 58, remission phase group 157 ± 34 normal controls 95 ± 26） （ P 〈0.01）. The combining capacity of CRTH2 and the total combining capacity on EOS in exacerbation phase group were significantly higher than those in remission phase group and normal controls（fmol/10^6 cell）（245.58± 48.27 vs 154.13 ± 67. 25,119. 29±53. 47 and 456.14±25. 57,337. 51±38. 52,301. 22±39. 54） （ P 〈0.01）. Conclusions The combining capacity of CRTH 2 on EOS in exacerbation phase group are significantly higher, this imply that PGD2 mediates the chemotaxis of EOS will be via CRTH2 receptor.
出处
《中华哮喘杂志（电子版）》
CAS
2009年第5期15-18,共4页
Chinese Journal of Asthma(Electronic Version)
基金
浙江省科技厅立项课题(编号:2006C33069)
金华市科技局立项课题(2004-1-361
2004-2-323)
关键词
哮喘
嗜酸粒细胞
CRTH2
Asthma
Eosinophil
Chemokine receptor-homologs molecule expressed on Th2 cells
