摘要
目的:观察解毒消癥饮含药血清抑制胃腺癌细胞增殖的相关因素。方法:采用血清药理学方法,MTT法检测含药血清对人胃腺癌细胞的增殖影响,采用化学比色法、ELISA、R IA检测解毒消癥饮含药血清的NO、NOS、TNF-α、IL-1β、VEGF和内毒素含量。结果:MTT实验结果显示,与胎牛血清对照组比较,含药血清组可以显著抑制胃腺癌细胞的生长,且呈时间剂量依赖;各组血清的内毒素水平均符合胎牛血清的质量标准;与空白组比较,NO、NOS、TNF-α、IL-1β有显著差异,各组VEGF的水平没有差异;而且NO、NOS差异与胃腺癌细胞增殖抑制率具有较高的相关性。结论:解毒消癥饮含药血清的NO、NOS水平与其药理作用密切相关,可作为其含药血清的质量标准之一。
Objective：To observe the related factors that Jiedu-Xiaozheng decoction contained serum inhibitted proliferation of human gastric gland carcinoma cells.Methods：Serologic pharmacology method and MTT assay were used to examine proliferating effect of serum containing Jiedu-Xiaozheng decoction on human gastric gland carcinoma cells,The chemical colorimetric method,ELISA and RIA were used to detect the contents of NO,NOS,VEGF,TNF-α,IL-1β and endotoxin in serum.Results：The MTT results showed that compared with control group,the serum groups could inhibit proliferation of gastric gland carcinoma cells significantly and had the time-doses relation.The level of endotoxin in all contained serum was equaled fetal bovine serum.Compared with control group,the contents of NO,NOS,TNF-α,IL-1β were significant,The content of serum VEGF had no significance.NO and NOS were higher related to inhibiting ratio.Conclusion：The serum NO and NOS levels were related to pharmacological action,that could be considered to one of contained serum qualitative criteria.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2009年第5期91-93,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
福建省教育厅A类科技项目(JA08122)
陈可冀中西医结合发展基金(CKJ2007005、CKJ2008060)
福建省自然科学基金(2009J01171)
关键词
解毒消癥饮
含药血清
胃腺癌细胞
一氧化氮
内毒素
细胞因子
Jieduxiaozheng Decoction（解毒消癥饮）
drug-containing serum
human gastric gland carcinoma cells
nitric oxide
endotoxin
cytokine
