摘要
目的：比较短毛柽柳中不同部位总黄酮的含量差异,研究黄酮化合物保存的条件,方法：采用超声波乙醇浸提法,在正交优先的最佳条件（料液比为1∶15,超声处理时间为40min,乙醇浓度为45%,超声温度50℃,提取3次）下提取不同部位总黄酮,并测定不同条件下其吸光度的变化。结果：柽柳花、叶、茎中总黄酮得率分别为24.585mg/g、25.623mg/g、41.726mg/g,柽柳中提取的黄酮溶液在光照、高温、高浓度蔗糖溶液和Na＋存在条件下不稳定;pH 4~7条件下较稳定;氧化剂对其稳定性的影响不大。结论：柽柳茎中总黄酮含量明显高于其他两个部位。柽柳黄酮保存时应避免接触碳水化合物和盐离子,在避光、常温、中性条件下保存。
Objective： To compare the content of total flavones in different parts of short-haired Tamarix karelini Bge,and study the preservative conditions of flavonoid compounds.Method： To apply ethanol extraction with Ultrasonic to extract total flavonoids of different parts,under the best priority orthogonal conditions（material to liquid＇s ratio 1∶15,ultrasonic time 40 mins,ethanol concentration 45%,ultrasound temperature 50℃,extracted 3 times）.and measured its absorbance under different conditions.Result：The yield of flower,browse and caudex isoflavones in the extracts respectively were 24.585mg/g,25.623 mg/g,41.726mg/g.The flavanone solution will be decompose action by high temperature and natural light irradiate directly;it is unstable around metalion of Na＋ and higher sucrose condition;the pH value of flavanone solution are stable well between pH 4 and 7;and that is stable in oxidant of H2O2.Conclusion： The flavonoids content in caudex is apparently higher than other two regions,This method is a purely physical process,and has not any pollution;it is an ideal way to extract the flavanone of Tamarix karelini Bge.
出处
《生物技术》
CAS
CSCD
北大核心
2009年第6期32-34,共3页
Biotechnology
关键词
短毛柽柳
黄酮化合物
超声波法
稳定性
正交试验
Tamarix karelini Bge. flavonoids ultrasonic wave treatment stability orthogonal experiment
