摘要
目的：研究建立胡椒叶片中提取高质量DNA的方法。方法：采用各种CTAB法和SDS法的改良方法,提取胡椒叶片中的总DNA,并对DNA进行紫外和电泳检测。结果：改良CTAB法4和5提取的DNA经电泳检测,有一条明亮主带,且无拖尾现象,样品槽无荧光出现,说明抽出的DNA纯度较高,一致性好;测定其OD260和OD280值,并计算其比值,OD260/OD280值在1.8-2.0之间,提取率在51.667-60.000μg/g之间,获得的基因组DNA质量高。结论：改良CTAB法4和法5可从胡椒幼叶中提取高质量DNA。
Objective： To establish the optimum method for extracting high-quality DNA from leaves of black pepper（Piper nigrum L.） that are rich in polysaccharides,polyphenols and other secondary metabolites.Method： Several improved CTAB methods and improved SDS methods were used to extract total DNA from young leaves,and the quality of DNA was detected by UV scanning and electrophoresis.Result： The DNA sample in the gel had a bright band,without smearing and non-fluoresce present in the gel wells with the modified CTAB method 4 and 5.The ratio of OD260/OD280 was between 1.8-2.0 and the extraction rate of DNA was between 51.667-60.000μg/g.Conclusion： The improved CTAB method 4 and 5 are suitable for extracting high-quality DNA from young leaves of black pepper（Piper nigrum L.）.
出处
《生物技术》
CAS
CSCD
北大核心
2009年第6期41-44,共4页
Biotechnology
基金
海南省重点学科建设项目("热带植物资源开发与利用"子项目"热带作物新品种选育及推广应用"子课题"华南胡椒种质资源收集
鉴定与细胞工程育种")资助
关键词
胡椒
DNA提取
改良CTAB法
改良SDS法
black pepper Piper nigrum L. DNA extraction improved CTAB method improved SDS method
