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人DNAJB6蛋白与人巨细胞病毒皮层蛋白pUL23相互作用的鉴定 被引量：6

Identification of Human DNAJB6 as an Interacting Protein for HCMV Tegument Protein pUL23
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摘要 pUL23是人巨细胞病毒(HCMV)UL23基因编码的皮层蛋白.HCMV皮层蛋白与病毒颗粒的形成、病毒转移、免疫调控等病毒生活过程相关.利用GAL4酵母双杂交系统筛选人胚肾cDNA文库,获得与人巨细胞病毒皮层蛋白pUL23相互作用的宿主蛋白分子DNAJB6[DnaJ(Hsp40)homolog,subfamily B,member 6].回复酵母双杂交、体外GST-Pull down和免疫共沉淀试验再次确认两者之间的相互作用.该结果为进一步研究pUL23蛋白在HCMV生活周期中的作用机制提供依据. Human cytomegalovirus（HCMV） is an opportunistic human pathogen that can cause life-threatening diseases in immunologically immature or compromised individuals,including neonates,AIDS patients and transplant recipients.UL23 gene encodes a tegument protein（pUL23） about 33 kD,of which the role has been implicated in the formation,transport,immunomodulation and transactivation of HCMV.To investigate the underlying function of UL23 during HCMV infections,we screened a human fetal kidney cDNA library using the GAL4 yeast two-hybrid assay.One of the positive clones was identified as the DNAJB6 [DnaJ（Hsp40） homolog,subfamily B,member 6].The interaction between DNAJB6 and pUL23 was further confirmed by GST-pull down and co-immunoprecipitation（Co-IP） experiment with cos-7 cells co-transfected with two plasmids containing DNAJB6 and pUL23.These results may provide important information to the understanding of pUL23 in the HCMV life cycle.
作者 姚伙旺 李实骞 胡嘉淼 陈业志 邹奕 张欣 李月琴 周天鸿 李弘剑
机构地区 暨南大学生命科学技术学院生物工程学系
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2009年第12期1143-1148,共6页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家自然科学基金项目(No.30770106) 国家自然科学基金重大研究计划面上项目(No.90608024) 广东省自然科学基金项目(No.06025162)~~
关键词 皮层蛋白pUL23 人巨细胞病毒 酵母双杂交 蛋白质相互作用 DNAJB6 tegument protein（pUL23） human cytomegalovirus yeast two-hybrid protein interaction DNAJB6
分类号 Q93 [生物学—微生物学] Q25 [生物学—细胞生物学]
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参考文献20

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共引文献9

同被引文献84

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引证文献6

二级引证文献4

中国生物化学与分子生物学报
2009年 第12期

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