摘要
本文研究了6-BA、IAA、NAA、IBA不同浓度处理对黄瓜外植体器官分化及植株再生的影响。结果表明:6-BA单独处理即可诱导外植体芽分化,浓度为0.5mg/L时分化率最高为33.3%,三种外植体中子叶远轴端无芽分化。IAA、NAA、IBA单独处理均可诱导外植体产生愈伤组织和不定根。两激素配比表明,MS+0.8mg/L 6-BA+0.10mg/L IAA最有利于子叶近轴端分化出芽,MS+0.8mg/L 6-BA+0.15mg/L IAA有利于下胚轴分化出芽,黄瓜植株再生率达30%。
The effect of different 6-BA、IAA、NAA and IBA concentration on explant differentiation and plant regeneration of cucumber was investigated in this experiment.The results were as follows： 6-BA can induce adventitious roots separately,especially in 0.5mg/L rates reached 33.3%.Among the three explants,far hypocotyledonary cotyledon explants couldn＇t induce adventitious roots.IAA、NAA、IBA could induce callus and adventitious roots separately.High frequency could be obtained in experiments of two hormones combination,especially in 0.8mg/L MS＋6-BA＋0.10mg/L IAA and 0.8mg/L MS＋6-BA＋0.15mg/L IAA,from near hypocotyledonary cotyledon explants and hypocotyl explants.The cucumber plant regeneration reached 30%.
出处
《安徽科技学院学报》
2009年第6期23-27,共5页
Journal of Anhui Science and Technology University
基金
安徽科技学院引进人才专项(ZRC2007101)
关键词
黄瓜
愈伤组织
不定芽
不定根
植株再生
Cucumber
Callus
Adventitious Buds
Adventitious Roots
Plant Regeneration
