摘要
目的观察氟对大鼠甲状腺形态、甲状腺过氧化酶(TPO)活性及血清甲状腺激素的影响。方法断乳1月龄的SD大鼠随机分为对照组、低氟组、中氟组、高氟组,分别饮用不同氟浓度的水,饲养6个月后处死。摘取甲状腺,在光镜下观察甲状腺形态结构的变化;改良愈创木酚法测定TPO活性;采用放射免疫方法测定血清甲状腺激素水平。结果高氟组有部分滤泡明显增大,滤泡腔内充满浓染胶质。低氟组、中氟组、高氟组各组随着染氟剂量增加,甲状腺过氧化酶(TPO)活性与对照组相比明显下降(P<0.05)。高氟组FT4水平与对照组相比显著降低(P<0.05),有统计学意义。结论长期摄入过量氟可造成甲状腺组织学改变,抑制TPO活性,从而造成甲状腺激素合成降低,由此可知,氟化物可引起甲状腺肿大,并导致甲状腺代谢功能异常。
Objective To observe the effects of fluoride on thyroid morphology, thyroid peroxidase and serum thyroid hormones. Methods One -month ablactating SD rats were randomly divided into three groups： the control group, low -fluoride group, middle - fluoride group, high - fluoride group, fed with water containing different fluoride concentration by adding NaF respectively. Rats were sacrificed after being fed for six months. The morphology of thyroid was observed through light microscope. The TPO activity was measured with upgrade guaiacol method. Radio - immunoassay was used to detect serum thyroid hormones. Results The major chan- ges included increased follicles with colloid accumulation in high fluoride groups. With the dose of fluoride increasing, TPO activity significantly decreased as compared with the control group （ P 〈 0. 05 ）. FF4 levels of the high - fluoride were significantly lower com- pared with the control group （ P 〈 0. 05）. Conclusions Chronic fluoride excess leads to definite histological changes in rat thyroid, inhibiting TPO activity so that level of thyroid hormone is decreased, which shows that fluoride can cause goiter, and cause abnormal changes of thyroid metabolism function.
出处
《辽宁医学院学报》
CAS
2009年第5期407-409,479,共4页
Journal of Liaoning Medical University (LNMU) Bimonthly
基金
辽宁省教育厅资助项目
编号:05L133
