摘要
目的研究子宫内膜息肉(EP)治疗前后雌激素受体(ER)、孕激素受体(PR)及热休克蛋白70、90(HSP70、90)的表达及意义。方法采用半定量逆转录聚合酶链反应(RT-PCR)来检测29例EP及其治疗后3～6个月后内膜中息肉中ER、PR、HSP70和HSP90的转录水平。结果治疗后子宫内膜中ER、HSP70较治疗前EP组织中表达降低(P<0.05),而PR和HSP90改变不明显(P>0.05)。结论通过刮宫治疗EP,能够使子宫内膜内环境得到一定改善,ER、HSP70在本病发生中起重要作用。
Objective To investigate the role of estrogen receptor（ER）,progesterone receptor（PR）,heat shock protein 70 and 90（HSP70,90）in endometrial polyps（EP）by detecting their expression before and after treatment.Methods 29 cases of endometrial polyps were obtained from the patients who underwent a surgical treatment by hysteroscopy（transcervical resection of endometrial polyps,TCRP）.The endometrial tissue of the same person was obtained in 3 to 6 month after the operation.The expression of mRNA of ER,PR,HSP70 and HSP90 was detected by reverse transcriptase PCR（RT-PCR）in differdnt groups.Results The expression of ER and HSP70 after treatment was significantly lower than that in endometrial polyps（P〈0.05）.But there was no significant difference in the changes of expression of PR and HSP90 before and after treatment（P〉0.05）.Conclusion TCRP is effective in treating endometrial polyp,and ER,HSP70 may play an important role in the pathogenesis of endometrial polyps.
出处
《河北医药》
CAS
2009年第24期3343-3345,共3页
Hebei Medical Journal
基金
河北省科学技术研究与发展指导计划项目(编号:04276101)
