摘要
目的探讨肿瘤坏死因子-α(TNF-α)促进滑膜细胞增殖的可能机制。方法将常规培养大鼠滑膜细胞株RSC-364细胞随机分为正常对照组和10μg/LTNF-α刺激组,分别培养6、12、24h,流式细胞术检测cyclinD1/CDK4蛋白表达;免疫细胞化学检测PCNA蛋白表达。结果TNFα作用6～24hcyclinD1和CDK4蛋白相对表达量逐渐增加,与正常对照组相比差异有统计学意义(P<0.01);PCNA蛋白阳性信号均表达于滑膜细胞核内,随着作用时间延长,PCNA蛋白表达增强,阳性细胞数目呈时间依赖性增多。cyclinD1和CDK4蛋白表达与PCNA的表达呈正相关(r值分别为0.683和0.496,P<0.01)。结论TNFα可能通过上调细胞周期调控蛋白cyclinD1/CDK4的表达促进滑膜细胞的增殖和分化。
Objective To investigate the effect and possible mechanism of tumor necrosis factor（TNF）α in promoting proliferation process of RSC-364 synoviocytes.Methods RSC-364 cells were cultured in vitro and divided into control group and induction group induced by 10μg/L TNF-α,then the cells were collected in 6h,12h,24h respectively.The expression of Cyclin D1/CDK4 proteins were detected by flow cytometry,and the expression of PCNA was detected by immunocytochemistry（ICC）staining.Results The expression of Cyclin D1 and CDK4 were significantly up-regulated by TNF-α,as compared with that in control group,there was a significant difference betweem two groups（P〈0.01）.ICC staining showed that the positive signal expression of PCNA located in nucleus.The number of positive cells of PCNA increased in a time-dependent mode.The Cyclin D1/CDK4 protein expression was closely correlated with PCNA expression.Conclusion TNF-α can promote the proliferation of RSC-364 cells by up-regulating the protein expression of CyclinD1/CDK4.
出处
《河北医药》
CAS
2009年第24期3350-3351,共2页
Hebei Medical Journal
基金
河北省科学技术研究与发展攻关基金资助项目(编号:08276101D-16)
