摘要
目的探讨海鞘醇衍生物002(SC002)抗肿瘤机制。方法应用酶联免疫吸附实验(ELISA法)检测SC002对荷瘤小鼠血清中血管内皮细胞生长因子(VEGF)含量的影响;采用鸡胚绒毛尿囊膜实验(CAM模型法)检测不同剂量SC002对新生血管的抑制作用。结果SC002可降低荷瘤小鼠血清中VEGF的含量;抑制肿瘤血管生成实验的形态学检测结果表明,SC002对新生血管的生长有抑制作用,SC002中剂量组对给药载体直接接触的CAM膜上和膜周围的新生血管生长抑制作用最明显。结论SC002可能通过降低荷瘤小鼠模型血清中促血管生成因子VEGF含量和直接抑制肿瘤新生血管生成两条途径发挥抗肿瘤活性,这一发现将为SC002作为新型的抗肿瘤药物提供实验依据。
Aim To study the anti-tumor mechanisms of SC002. Methods Following the treatment of the tumor-challenged mice with SC002, the changes in serum of tumor associated cell signal molecule VEGF （ vascular endothelial growth factor） were observed by ELISA while using the chick chorioallantoic membrance（ CAM ） method the inhibitory action of different doses of SC002 was observed in vivo.Results ELISA showed that SC002 could obviously reduce the concentration of serum VEGF in tumor-bearing mice,the middle dosage group of SC002 could suppress angiogenesis on the directly-contacted chorioallantoic membrane or around them.Conclusion SC002 has anti-tumor function by suppressing the expression of VEGF.This finding is theoretically important for its future use as a novel anti-tumor drug.
出处
《解放军药学学报》
CAS
2009年第6期485-488,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家自然科学基金面上项目
No.30771969
