摘要
以肉色香蘑子实体为材料,采用不同有机溶剂萃取子实体干粉,除多糖外,各有机相提取物的得率为:乙醇相>石油醚相>乙酸乙酯相。以叔丁基羟基茴香醚(BHA)为阳性对照,通过抑制1,1-二苯代苦味肼基(DPPH)自由基实验和清除超氧阴离子自由基实验,对各提取物的体外抗氧化活性进行测定,结果显示:各有机相提取物和多糖均具有不同程度的抗氧化活性,以乙酸乙酯相、乙醇相提取物的抗氧化活性最强。以抗肿瘤药物顺铂为阳性对照,采用MTT法对各提取物的体外抗肿瘤活性进行检测,结果表明:各相提取物均具不同程度的抗肿瘤活性,其中乙酸乙酯相的抗肿瘤活性最强,达到82.7%,显著(P<0.05)高于阳性对照;其次为多糖,同浓度下与顺铂较接近。综上所述,肉色香蘑子实体含丰富的抗氧化、抗肿瘤活性成分。
The dried powder of fruit body of Lepista irina （Fr.） Bigelow was extracted with petroleum ether （PE）, ethyl acetate （EA）, ethanol （Eth） and polysaccharides were prepared. The yield of various extracts was 7.284% （Eth-portion）, 2.284% （PE-portion）, 0.858% （EA-portion） and 4.107% （polysaccharides）, respectively. The antioxidant and antitumor properties of solvent extracts were studied by free radical scavenging assay and MTT assay. The results showed that all extracts and polysaccharides provided various antioxidant activities against radicals of DPPH and hydroxyl and EA and Eth extracts possessed the highest effect. MTT data suggest the varying antitumor activities of extracts and the EA extract showed the highest antiproliferative effect （82.7%）. The polysaccharide followed the second and showed higher antitumor activity equivalent to cisplatin. In summary, the mushroom is a promising and potential source for screening natural antioxidants and antitumor agents.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第23期214-217,共4页
Food Science
基金
河北省自然科学基金项目(C2008000155)
河北省教育厅科学研究重点项目(ZH2007107)
