摘要
为了探讨葛花对急性酒精中毒的防治作用,将雄性小鼠随机分为空白组、模型组、葛花提取物组。给药30min后,模型组与葛花组灌酒0.10mL/10g^-1,在灌酒后0.5,1,1.5,2,2.5,3h分别摘眼球取血和取肝组织。采用生化酶法测定小鼠酒后不同时间内血中乙醇浓度和肝中乙醇脱氢酶（ADH）活性。结果显示,小鼠血中乙醇浓度在酒后0.5-2h达到高峰,与模型组相比,葛花提取物组0.5-3h内的血中乙醇浓度数值降低。葛花提取物组对小鼠酒后0.5-1h肝中ADH的影响较为明显,使其活性增加;酒后1.5-3h肝中ADH活性虽然也增强,但与模型组比较无显著差异。结果显示,葛花提取物可降低酒后血中乙醇浓度,增强肝中ADH活性,其机制可能是通过抑制消化道对乙醇的吸收,从而起到有效的降醇解酒作用。
To observe the effect of Pueraria lobata （Willd.） Ohwi on relief of alcohol toxicity by animal experiments, female NIH mice were ovariectomized and randomly divided into 5 groups. The animal models of acute alcoholism were duplicated by force-feeding them with liquor ＂er guo tou＂. The influence of aqueous extract of Pueraria lobata （Willd.） Ohwion on the alcohol concentration in blood within 0.5 -3 hours was studied by methods of biochemistry. The alcohol concentration in blood took the highest value in 0.5-1.5 hours. Compared to the model group, the alcohol concentration in blood for the group of aqueous extract of Pueraria lobata （Willd.） Ohwi is decreased in 0.5-3 hours; the activity of ADH in the liver is increased in 2-3 hours （P〉0.05）, while it is significantly increased in the first 0.5-1 hours （P〈0.05）. The aqueous extract of Pueraria lobata （Willd.） Ohwi could reduce the alcohol concentration in blood of animals after being fed with alcohol and increase the activity of ADH. It relieves alcohol toxicity and prevents drunkenness by restraining the absorption of alcohol in the gastrointestinal tract and promoting the metabolism of alcohol in the liver.
出处
《科技导报》
CAS
CSCD
北大核心
2009年第23期82-85,共4页
Science & Technology Review
关键词
葛花
酒精中毒
解酒
乙醇浓度
乙醇脱氢酶活性
Pueraria lobata （Willd.） Ohwion
alcoholism
relieving alcohol
alcohol concentration
activity of alcohol dehydrogenase
