摘要
目的了解天津产ESBLs大肠埃希菌、肺炎克雷伯菌基因型及耐药情况。方法收集部分医院临床分离218株产ESBLs大肠埃希菌、肺炎克雷伯菌,利用实时荧光定量PCR方法检测CTX-M-1、CTX-M-2、CTX-M-8、CTX-M-9、TEM、SHV、OXA-1、OXA-2、OXA-10耐药基因,结合Tm值将其分型。结果109株产ESBLs大肠埃希菌耐药基因CTX-M-1、CTX-M-2、CTX-M-8、CTX-M-9、TEM、SHV、OXA-1分别为32.1%、0.9%、0.9%、33.9%、73.4%、27.5%、15.6%,携带>2种基因菌株达66.1%;109株产ESBLs肺炎克雷伯菌耐药基因CTX-M-1、CTX-M-2、CTX-M-8、CTX-M-9、TEM、SHV、OXA-1、OXA-10分别为49.5%、2.8%、1.8%、22.9%、78.9%、76.1%、33.0%、9.0%,携带>2种基因达82.2%;检测菌株对亚胺培南保持高度敏感。结论天津市肺炎克雷伯菌及大肠埃希菌ESBLs的基因型以SHV型、TEM型、CTX-M-1型、CTX-M-9型为主,OXA呈上升趋势,各医院肺炎克雷伯菌耐药基因型存在一定差异。
OBJECTIVE To investigate the genotype characteristics of extended-spectrum β-lactamases（ESBLs） producing Escherichia coli and Klebsiella pneumoniae and drug resistance condition in Tianjin.METHODS A total of 218 strains of ESBLs-producing E.coli and K.pneumoniae were isolated from clinic.The drug-resistant CTX-M-1,CTX-M-2,CTX-M-8,CTX-M-9,TEM,SHV,OXA-1,OXA-2 and OXA-10 were tested by SYBR GREEN Ⅰ real-time fluorescent quantitative Polymerase chain reaction（PCR）.RESULTS Among 109 strains of ESBLs-producing E.coli,the drug resistance of genotypes CTX-M-1,CTX-M-2,CTX-M-8,CTX-M-9,TEM,SHV and OXA-1 was 32.1%,0.9%,0.9%,33.9%,73.4%,27.5% and 15.6%,respectively.The strains with more than two genes were 66.1%.The positive rate of CTX-M-1,CTX-M-2,CTX-M-8,CTX-M-9,TEM,SHV,OXA-1 and OXA-10 was 49.5%,2.8%,1.8%,22.9%,78.9%,76.1%,33.0% and 0.9%,respectively.The strains with more than two genes were 82.2%.The strains were highly sensitive to imipenem.CONCLUSIONS Most of the genotypes of ESBLs-producing E.coli and K.pneumoniae in Tianjin are SHV,TEM,CTX-M-1 and CTX-M-9.The rate of OXA are increasing,and the drug resistance genes of K.pneumoniae from different hospitals are different.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第24期3321-3324,共4页
Chinese Journal of Nosocomiology
基金
天津市卫生局基金资助项目(02KY38)
