摘要
目的:研究周期性张应变对人牙髓细胞形态、存活率和增殖活性的影响。方法:取新鲜拔除的健康恒牙牙髓,采用组织块贴壁法培养原代人牙髓细胞,通过Flexcell细胞应力培养系统,分别加载2%和8%的周期性张应变,加载频率为1Hz,加载时间分别为0.5h、12h及24h,以未加载的静态细胞作为对照组。应用倒置相差显微镜、台盼蓝法及MTT法分别检测细胞形态、存活率和增殖活性的变化。数据采用SPSS16.0软件包进行单因素方差分析。结果:张应变加载后,贴壁的牙髓细胞形态为长梭形,有明显的极性突起,并且排列趋向一致,加载24h变化更明显。2%、8%加载组细胞的存活率显著大于对照组,12h加载组细胞存活率最高,24h加载组略有下降。2%、8%加载组细胞的增殖能力与对照组相比增加,2%组加载24h增加最明显(P<0.01)。结论:周期性张应变对离体培养的人牙髓细胞形态、存活率和增殖活性有明显影响,并呈应变大小和时间依赖性。
PURPOSE：To investigate the effect of mechanical strain on cell morphology,viability and proliferation of human dental pulp cells in vitro.METHODS：Human dental pulp cells were cultured and subjected to 2% or 8% strain with Flexcell Tension Plus System for 0.5 hour,12 hours and 24 hours,respectively,and then the cell morphology,viability and proliferation were examined by phase contrast microscope,trypan-blue staining and MTT method.The results were analyzed by one-way ANOVA with SPSS16.0 software package.RESULTS：Cells were stretched and aligned perpendicular to the direction of the force applied with obvious pole formation under the tested condition.The viability and proliferation of the cells subjected 2% or 8% strain were significantly higher than that of untreated cells,which reached the peak at 12 hours.The proliferation of the cells increased after loading strain which was significantly higher than that in the control by 2% stain subjected for 24 hours （P〈 0.01）.CONCLUSIONS：Cyclic strain could affect morphology,viability and proliferation of in vitro cultured human dental pulp cells in a magnitude/time dependent manner.
出处
《上海口腔医学》
CAS
CSCD
2009年第6期599-603,共5页
Shanghai Journal of Stomatology
基金
国家自然科学基金(30471889)~~
