摘要
通过比较急性白血病患者治疗前后骨髓中NK细胞活性和数量的变化,探讨其在白血病治疗中的意义和白血病治疗的免疫学方法。用淋巴细胞分离液分离骨髓和外周血中单个核细胞,用MTT法测定NK细胞的活性,用流式细胞仪测定NK细胞的数量。结果显示:30例初发组白血病患者NK细胞活性[(13.52±5.82)%]和数量[(6.63±2.95)%]与缓解组NK细胞活性[(30.50±9.67)%]和数量[(13.57±4.48)%]相比均明显降低,两者存在统计学差异(P<0.01)。结论:急性白血病患者完全缓解后骨髓中NK细胞的活性和数量均明显提高。
To compare the changes of activity and quantity of nature killer（NK） cells in bone marrow of patients with acute leukemia between before and after treatment in order to explore the significance of immunologic therapy methods in leukemia treatment.The mononuclear cell of bone marrow was separated and the activity of NK cell was assayed by MTT,the quantity of NK cell was assayed by flow cytometry.The results showed that the quantity（6.63±2.95 %）and activity（13.52±5.82 %）of NK cells in 30 patients with acute leukemia before chemotherapy were significantly lower than that of complete remission group, which had quantity （13.57±4.48% ）and activity（30.50±9.67 % ）of NK cells （P 〈0.01 ）. The results indicate that the quantity and activity of NK cell in acute leukemia after complete remission are significant increased.
出处
《标记免疫分析与临床》
CAS
2009年第6期358-360,共3页
Labeled Immunoassays and Clinical Medicine
关键词
急性白血病
骨髓
自然杀伤细胞
活性
数量
Acute leukemia
Bone marrow
Natural kill cell
Activity
Quantity
