摘要
目的:探索β2肾上腺素受体(ADRB2)基因G1023A,α上皮细胞钠通道(ENaC)基因G2139A和G蛋白β3亚基(GNB3)基因C825T多态性与新疆维吾尔族人群原发性高血压的关系。方法:采用人群为基础的病例对照研究,高血压组269例,正常血压组229例。用聚合酶链反应一限制性片断长度多态性(PCR-RFLP)的方法检测ADRB2基因G1023A基因型、ENaC基因G2139A基因型和GNB3基因C825T基因型,分析其与与维尔族人群高血压的关系。结果:高血压组和正常血压组的ADRB2基因型分布分别为GG:0.202、0.147;AG:0.266、0.339;AA:0.532、0.513,差异无统计学意义(P=0.119)。高血压组和血压正常组的ENaC基因型分布分别为GG:0.249、0.266;AG:0.472、0.476;AA:0.281、0.260,差异无统计学意义(P=0.840);高血压组和血压正常组的GNB3基因型分布分别为CC:0.346、0.293;CT:0.394、0.437;TT:0.260、0.271,差异无统计学意义(P=0.418)。用Logistic回归模型校正年龄、性别、吸烟、体重指数、腰围、臀围、血糖、甘油三酯、总胆固醇及胆红素之后,亦未发现三个位点与原发性高血压有关。进一步分析未发现三个位点对维吾尔族高血压的发病有交互作用(P=0.080)。结论:在新疆维吾尔人群中,未发现ADRB2基因G1023A、ENaC基因和GNB3基因多态性单独或联合作用在高血压发病中起作用。
Objective ：To investigate the relationship between polymorphisms of G1023A in ADRB2 gene, G2139 A in ENaC gene and C825T in GNB3 gene and essential hypertension occurrence in Uygur Population. Methods： Population based case-control study was designed into two groups, Essential hypertension （Hypertension）group, n = 269, the patients with the systolic pressure ≥ 140 mmHg and/or diastolic pressure≥90 mmHg, and Control group, n = 229, the subjects with the systolic pressure 〈 140 mmHg and the diastolic pressuer 〈 90 mmHg. ARDB2 gene G1023A locus,ENaC gene G2139A, and GNB3 gene C825T polymorphisms were examined by PCR-RFLP method. Resuhs ： The frequencies of ADRB2 genotypes of GG, AG, and AA were 20. 2% , 26.6. %, and 53.2% in Hypertension group,and 14.7% ,33.9% and 51.3% in Control group respectively,no significant difference（P =0. 119）. The frequencies of ENaC genotypes of GG,AG and AA were 24.9% 47. 2% and 28. 1% in Hypertension group,while 26. 6% ,47.6% ,26.0% in Control group respectively, no significant difference （ P = 0. 840 ）. The frequencies of GNB3 genotypes of CC, CT, and TT were 34. 6% ,39.4% ,and 26. 0% in Hypertension group,and 29. 3% ,43.7% and 27. 1% in Control group respectively,no significant difference（P = 0. 080）. After adjustment of age, gender, smoking, body mass index, waistline, hip, triglyeefide, total cholesterol and total bilirubin by Logistic regression analysis, no association was found between each of three locus and the essential hypertension occurrence. Conclusion：The polymorphisms of G1023A in ADRB2 gene,G2139A in ENaC gene and C825T in GNB3 gene did not have obvious effect on essential hypertension occurrence in Uygur population.
出处
《中国循环杂志》
CSCD
北大核心
2009年第6期446-450,共5页
Chinese Circulation Journal
