摘要
目的：利用激光扫描共聚焦显微镜（ＬＳＣＭ）观察ＩＬ－１β及ＴＳＨ对鼠甲状腺ＦＲＴＬ－５细胞内Ｃａ２＋的影响，以进一步了解ＩＬ－１β对甲状腺细胞的作用及机制。方法：将待测ＦＲＴＬ－５细胞以Ｆｌｕｏ－３负载，以ＬＳＣＭ扫描测定细胞内Ｃａ２＋，分别加入不同浓度的ＴＳＨ或ＩＬ－１，观察细胞内Ｃａ２＋变化。结果：（１）ＴＳＨ可使细胞内Ｃａ２＋的水平上升，与ＴＳＨ浓度有关。（２）ＩＬ－１β可降低ＦＲＴＬ－５细胞内基础Ｃａ２＋和ＴＳＨ刺激的细胞内Ｃａ２＋的水平。结论：ＴＳＨ对甲状腺细胞磷酸肌醇／钙离子系统有刺激作用，Ｃａ２＋作为第二信使可介导ＴＳＨ的效应。
Objective:In order to investigate the effect of IL 1 β on the inositol phosphate (Inos)/Ca 2+ cascade in thyroid cells, the changes of cytosolic Ca 2+ induced by IL 1 β and TSH were observed by laser scanning confocal microscope(LSCM) in rat thyroid FRTL 5 cells.Methods:The cultured FRTL 5 cells loaded with Ca 2+ probe Fluo 3 were exposed to the different concentrations of TSH or IL 1β and then the cytosolic Ca 2+ were measued by LSCM. Results:The intracelular Ca 2+ levels of FRTL 5 cells were increased after adding TSH(1 to 5U/L) by dose dependent manner; while IL 1β decreased the basal levels and the TSH stimulated effect of cytosolic Ca 2+ . Conclusion:TSH may elevate cytosolic Ca 2+ levels, indicating that Ca 2+ can mediate the action of TSH on thyroid cells; IL 1β has a direct inhibitory effect on Inos/Ca 2+ cascade in thyroid cells.
出处
《军医进修学院学报》
CAS
1998年第4期270-271,共2页
Academic Journal of Pla Postgraduate Medical School
关键词
受体
白细胞介素1
促甲状腺素
钙
receptors, interleukin 1
thyrotropin
calcium/metabolism
ion channels
thyroid gland/cytology
