摘要
目的研究不同时程慢性束缚水浸应激对大鼠胃排空的影响。方法48只雄性150 g左右SD大鼠被随机分为6组,即实验3、72、8 d组和对照3、72、8 d组,每组8只。实验期间,每日观察大鼠体重、一般状况及行为学指标。实验组23℃水域箱内束缚水浸1 h/d,对照组自由摄食饮水。采用半固体排空实验(阿拉伯胶炭糊)来检测胃排空率,数据录入SPSS 13.0进行分析处理。结果①体重增加情况:不同组别和不同应激时间这两个因素对体重增加的影响差异均有显著性。②胃排空率:对照各组间比较均无统计学差异。3 d组间(实验3d组与对照3 d组)大鼠胃排空率无差异;而7 d组间和28 d组间比较则明显异常。随着应激时间的延长,实验组胃排空率呈现了先增高(7 d时)后降低(28 d)的变化趋势,且实验3 d组与7 d组、7 d组与28 d组间比较均有明显的统计学意义,而3 d组与28 d组比较则无统计学差异。结论不同时程的慢性束缚水浸应激可以导致大鼠胃排空率改变;随着应激时间的延长,呈现先上升后下降的规律。
Objective To study the effect of restraint and watered stress of different durations on the gastric empting in rats.Methods 48 male SD rats（weight ±150 g） were divided randomly into 6 groups with 8 in each.The experimental rats were restrained and watered in 23℃ water box for 1 h/d,while the controls were free.The body weight,general conditions and behavioral indexes of the rats were observed and recorded.The gastric empting rate was measured in rats with semi-solid diet after 3 d,7 d and 28 d during the experiment,respectively.Results ① The profile of weight gain： there were statistically significant differences among different groups of different stress durations;② The gastric empting rate： No difference between all the control groups,and between the 3 d test group and 3 d control group.The gastric empting rate was higer in the 7-day test than in 7 d controlled group,lower in the 28 d test group than 28 d control group.Conclusion Different chronic stress can cause changes of gastric empting rate.With the prolonging of stress time,the empting rate is rising and then decending.
出处
《中国实验动物学报》
CAS
CSCD
2009年第6期415-418,共4页
Acta Laboratorium Animalis Scientia Sinica
关键词
慢性束缚水浸应激
胃排空
大鼠
Chronic restraint
Watered stress
Gastric emptying： Rats
