摘要
目的初步探讨MLVA（multiple—locus variable—number tandem-repeat analysis）技术在黄疸出血群钩端螺旋体基因分型中的应用。方法选取7个VNTR位点，对我国致病性钩端螺旋体黄疸出血群菌株提取基因组DNA，采用PCR扩增和琼脂糖凝胶电泳技术检测，应用BioNumerics（Vetsion4．0）软件进行聚类分析。结果共对117株钩端螺旋体的7个VNTR位点进行了检测，聚类分析分为3个群（A群、B群、C群）28种基因型，其中A群占11．97％（14／117）、B群占0．85％（1／117）、C群占87．18％（102／117）；多态性指数介于0．0831与0．8005之间；MLVA基因型存在明显的地域性。结论MLVA分型技术可初步对钩端螺旋体进行遗传学分类鉴定，应用该技术，将在钩体病分子流行病学研究中发挥重要的作用。
Objective To establish the method of multiple loci VNTR（ variable numbers tandemrepeats） analysis （MLVA） for genotyping Leptospira interrogans serogroup Icterohaemorrhagiae . Methods Seven VNTR loci were chosen for genotyping 117 strains of L. interrogans serogroup Icterobaemorrhagiae by PCR-electrophoresis-based VNTR analysis and the results were analyzed by software BioNumerics （Version 4.0）. Results One hundred and seventeen isolates of L. interrogans serogroup lcterohaemorrhagiae detected with 7 VNTR loci were classified into three clusters （ A, B, C）, twenty-eight types were found, type A 11.97% （14/117）, type B 0.85% （ 1/117）, type C 87.18% （102/117）. Diversity Indexes for the loci varied between 0.0831 and 0. 8005. Clinical strains isolated from the same geographic area and belonging to the same serogroup shared a common VNTR pattern. Conclusion MLVA could be used to classify and identify Leptospira interrogans preliminarily. With the improvement of technology, this rapid and easy method should greatly contribute to a better knowledge of the epidemiology of Leptospira.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2009年第12期1144-1147,共4页
Chinese Journal of Microbiology and Immunology
基金
卫生行业科研专项项目（200802016）
国家863计划专项课题（2006AA02Z176）
关键词
钩端螺旋体
黄疸出血群
基因分型
MLVA
Leptospira interrogans
Serogroup Icterohaemorrhagiae
Genotyping
MLVA
