摘要
目的:探讨Ghrelin对胃外分泌功能的作用.方法:从断奶大鼠中获得新鲜胃黏膜组织.分离培养胃黏膜上皮细胞,培养30h后,实验组换为分别含有1×10-4、1×10-3、1×10-2和1×10-1μmol/LGhrelin的新鲜培养液,对照组换为不含Ghrelin的正常新鲜培养液.继续培养4h,收集培养液和细胞,分别测定细胞的相对活性(MTT法)、培养液中胃蛋白酶活性、细胞中H+-K+-ATPasemRNA表达和活性.结果:实验组中细胞的相对活性与对照组相比没有发生明显的变化;1×10-3μmol/LGhrelin可显著提高胃蛋白酶的活性(P<0.05).1×10-3μmol/LGhrelin可显著增加细胞中H+-K+-ATPasemRNA的表达(P<0.05),1×10-4和1×10-3μmol/LGhrelin明显增加了胃黏膜上皮细胞中H+-K+-ATPase的活性(均P<0.05).结论:Ghrelin体外作用于胃黏膜上皮细胞可刺激胃蛋白酶和胃酸的分泌.
AIM： To investigate the effects of ghrelin on gastric exocrine secretion. METHODS： Gastric mucosa was obtained from weaned rats. Gastric mucosal cells were dispersed from freshly obtained gastric mucosa and divided into two groups： treatment group and control group. The treatment group was incubated with fresh culture medium containing different concentrations of ghrelin （1×10^-4, 1×10^-3, 1×10^-2 and 1×10^-1 μmol/L）, while the control group was incubated with fresh culture medium without ghrelin. Four hours after incubation, culture supernatants were collected, and cells were harvested. Cell viability was measured by methyl thiazolyl tetrazolium （MTT） assay. Pepsin activity and H^＋-K^＋-ATPase mRNA expression and activity were evaluated. RESULTS： No significant difference was noted in cell viability between the control group and treatment group. Ghrelin at a concentration of 1×10^-3 μmol/L significantly augmented pepsin activity and H^＋-K^＋-ATPase mRNA expression （both P 〈 0.05）. Ghrelin at a concentration of lx10-4 and 1×10^-3 μmol/L significantly increased H^＋- K^＋-ATPase activity in gastric mucosal cells （both P 〈 0.05）. CONCLUSION： Ghrelin can significantly stimulate the secretion of both pepsin and gastric acid by gastric mucosal cells in vitro.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第34期3486-3490,共5页
World Chinese Journal of Digestology
基金
江苏省自然科学基金资助项目
No.BK2007536
南京市科技发展计划基金资助项目
No.20081b311085
2008年江苏省"青蓝工程"基金资助项目~~
