摘要
采用荧光极化免疫分析法，检测了１５例老年心功能不全病人血清地高辛样免疫活性物质（ＤＬＩＳ）浓度。以ＴＤｘ仪最低检测限０２５６ｎｍｏｌ·Ｌ－１为阳性检测标准，ＤＬＩＳ检出阳性率为４６７％（７／１５），浓度范围０２６～１５２ｎｍｏｌ·Ｌ－１，平均浓度０５５±０４４ｎｍｏｌ·Ｌ－１；其中１例病人血清ＤＬＩＳ浓度高达１５２ｎｍｏｌ·Ｌ－１。结果表明老年心功能不全患者血清中含有较高水平的ＤＬＩＳ；提示对这些患者若因病情需要而用地高辛治疗时，其后的血浓度测定结果易引起“过高估计”。
The presence of a higher concentration of digoxinlike immunoreactive substances(DLIS) in human serum have been reported in a number of pathophysiological conditions. DLIS can react with antidigoxin antibodies when determination of serum digoxin was performed with immunoassay. It may falsely elevate the serum digoxin concentration and is troublesome in the therapeutic monitoring of digoxin. The apparent digoxin concentrations(DLIS) in serum were determined in 15 elder patients with cardiac insufficiency by fluorescence polarization immunoassay(FPIA). The lowest measurable concentration, defined as the concentration that could be distinguished from zero with 95% confidence, was 0256 nmol·L-1. Steroids and drugs commonly administered with digoxin showed no significant crossreactivity. In these patients, the positive ratio for determination of DLIS was 467%(7 in 15 cases), and its mean serum concentration was 055±044 nmol·L-1 (range 026～152 nmol·L-1). The results were well consistent with that obtained by Dasgupta et al. In conclusion, there are certain increased concentrations of DLIS in the sera of elder patients with cardiac insufficiency, suggesting that digoxin levels measured by immunoassay method must be interpreted carefully in these patients after treatment with digoxin or other digitalis preparations.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第9期655-658,共4页
Acta Pharmaceutica Sinica
基金
国家人事部科研基金
关键词
地高辛
免疫活性物质
心功能不全
老年人
Digoxinlike immmunoreactive substances
Fluorescence polarization immunoassay
Digoxin
Cardiac insufficiency
