期刊文献+

复合酶法提取远志总皂苷的工艺研究 被引量:5

Extraction of Total Saponin in Radix Polygalae by the Conbimed Enzymolysis
下载PDF
导出
摘要 目的:研究酶法结合传统回流法提取远志总皂苷工艺条件。方法:利用可见分光光度法测定远志总皂苷含量;考察植物复合酶的酶解时间、酶解温度对传统提取工艺的强化效果;通过显微结构图和红外光谱图说明了复合酶法强化传统提取法的作用方式及提取物的结构。结果:酶用量为0.4%时,适宜酶解温度为55℃,最优酶解时间为0.5h。结论:复合酶法结合传统回流提取后,可提高远志总皂苷提取率、缩短提取时间、减少乙醇使用量,对于远志流浸膏的高效提取具有一定指导意义。 This work investigated the extraction of total saponin in Polygala using the enzymolysis combined with the traditional reflux. The content of total saponin in Radix Polygala was determined by a visible spectrometer, and the strengthed effect of enzymolysis time and enzymolysis temperature was studied on the tradition extraction process. The function of the combined enzymolysis-strengthened traditional extraction and the structure of the extracts were deter- mined by the infrared absorption spectrum and microstructure spectrum. The best quantity of enzyme was found to be 0.4% ,the enzymolysis temperature 55℃ ,and the optimal enzymatic treatment 2.5h. The results showed that this method can increase the extraction rate of total saponin in Polygalae, shorten the extraction time, and reduce the consumption of ethanol, thus helping the extraction of polygala liquidum.
机构地区 山西中医学院
出处 《世界科学技术-中医药现代化》 2009年第6期885-888,共4页 Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金 山西省卫生厅科技攻关计划项目(2008034):远志皂苷提取分离方法研究 负责人:王颖莉
关键词 远志总皂苷 复合酶法 显微结构 红外光谱 远志流浸膏 Total saponin in Radix Polygalae Combined enzymolysis Infrared absorption Extraction polygalae liquidum
  • 相关文献

参考文献9

二级参考文献67

共引文献168

同被引文献80

引证文献5

二级引证文献62

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部