摘要
用比浊法测定血小板聚集率,观察甲基莲心碱(Nef)体外给药对正常与高脂血的人、大鼠及家兔血小板聚集功能的影响。结果表明,Nef在体外抑制ADP、胶原、Adr诱导的人血小板聚集,呈明显剂量依赖性。健康人组,对ADP、Adr诱聚1min时的IC_(50)分别为59和36μmol/L;对ADP、Adr、胶原诱聚5min时的IC_(50)分别为42,89和110μmol/L。高脂血症患者组,Nef对ADP、Adr诱聚1min时的IC_(50)分别为45,38μmol/L;对ADP、Adr、胶原诱聚5min时的IC_(50)分别为36,27和16μmol/L。Nef 50、500μmol/L也能明显抑制ADP诱导的以正常或高脂饮食喂养大鼠的血小板聚集。Nef(0.3～300μmol/L)抑制ADP、胶原诱导的兔血小板聚集呈剂量依赖性。
The effects of neferine of platelet aggregation were studied by turbidimetry . The result suggested that Nef markedly inhibited ADP, Adr and collagen-induced human platelet aggregation in dose-dependent compared with control group in vitro. In normal human, the IC50 of Nef on the platelet aggregation at the first phase induced by ADP and Adr were 59 and 36 μmol/L, respectively; the IC50 of platelet aggregation at the second phase induced by ADP, Adr and collagen were 42, 89 and 110 μmol/L, respectively. In patients with hyperlipemia, the IC50 of Nef on the platelet aggregation at the first phase induced by ADP and Adr were 45 and 38 μmol/L, respectively; the IC50 of platelet aggregation at the second phase induced by ADP, Adr and collagen were 36, 27 and 16 /μmol/L, respectively. Nef 50 μmol/L or 500μmol/L also markedly inhibited ADP-induced platelet aggregation in the rats fed with normal or high-fat diet in vitro. Nef(0.3-300 μmol/L) inhibited rabbit platelet aggregation induced by ADP and collagen in dose-dependent. It is showed that neferine is a broad-spectrum platelet aggregation inhibitor.
出处
《中国药师》
CAS
1998年第2期51-53,共3页
China Pharmacist
关键词
甲基莲心碱
高脂血症
血小板聚集
Neferine
Hyperlipemia
Turbidimetry
Platelet aggregation
