摘要
目的评估抗真菌药物布替奈芬对眼部致病真菌的体外抗菌活性。方法实验研究。参照美国临床和实验室标准化研究所制定的M38-A方案，用微量液基稀释法对260株（15属29种）眼部分离真菌进行布替奈芬体外抗菌活性研究，并与那他霉素、二性霉素B及氟康唑进行对比。布替奈芬的实验浓度范围0，008～16．000mg／L，那他霉素和二性霉素B的实验浓度范围0．031—16．000mg／L，氟康唑的实验浓度范围0．5—256．0mg／L。那他霉素和二性霉素B取100％的真菌生长抑制为最低抑菌浓度（MIC），布替奈芬和氟康唑取75％的真菌生长抑制为MIC。应用SPSS13．0统计学软件对数据进行处理，采用描述性统计分析方法计算4种药物对实验菌的MIC均数、MIC范围、MIC50及MIC90结果布替奈芬、那他霉素、二性霉素B、氟康唑对镰孢菌属的MIC90分别为4、8、2、512mg／L；对曲霉菌属的MIC90分别为0．063、32．000、2．000、256．000mg／L；对互隔交链孢霉的MIC90分别为0．5、8．0、2．0、128．0mg／L；对新月弯孢霉的MIC90分别为0．125、2．000、0．500、4．000mg／L；对罕见眼部致病真菌的MIC。分别为1、4、1、256mg／L。结论布替奈芬对眼部分离真菌具有广谱抗菌活性，对曲霉菌属、互隔交链孢霉及新月弯孢霉的抗菌活性尤强，提示开发布替奈芬滴眼剂型对临床治疗真菌性角膜炎具有重要意义。
Objective To investigate antifungal activity of butenafine in comparison with that of natamycin, amphotericin B and fluconazole against ocular pathogenic filmentous fungi in vitro. Methods It was an experimental study. Susceptibility tests were performed against 260 isolates of ocular pathogenic filmentous fungi by broth dilution antifungal susceptibility test of filamentous fungi approved by the Clinical and Laboratory Standards Institute （CLSI） M38-A document. The isolates included Fusarium spp. （136）, Aspergillus spp. （ 98 ）, Alternaria alternata （ 9 ）, Curvularia lunata （ 3 ）, and unusual ocular pathogens （14）. Final concentration ranged from 0. 008 to 16. 000 mg/L for butenafine, from 0. 031 to 16. 000 mg/L for amphotericin B and natamycin, and from 0. 5 to 256. 0 mg/L for fluconazole. Following incubation at 35 ℃ for 48 h, minimal inhibitory concentration （MIC） was determined according to the CLSI M38-A document. For amphotericin B and natamycin, the MIC was defined as the lowest drug concentration that prevented any discernible growth. For butenafine and fluconazole, the MIC was defined as the lowest concentration in which an approximately 75% reduction compared to the growth of the control was observed. Candida parapsilosis ATCC22019 was used as quality control strains to validated the results. Mean MIC and MIC range, the MIC at which 50% of the isolates tested were inhibited （MIC50） and the MIC at which 90% of the isolates tested were inhibited （ MIC90 ）, were provided for all the isolates tested by using descriptive statistical analysis with the statistical SPSS package （version 13.0 ）. Results MIC90 of butenafine, natamycin, amphotericin B and fluconazole were 4,8,2 and 512 mg/L for Fusarium spp. , respectively; 0. 063, 32. 000, 2. 000 and 256. 000 mg/L for Aspergillus spp. , respectively; 0. 5, 8.0, 2.0 and 128.0 mg/L for Alternaria alternat, respectively; 0. 125, 2. 000, 0. 500 and 4. 000 mg/L for Curvularia lunata, respectively; and 1,4, 1 and 256 mg/L for unusual ocular pathogens, respectively. Conclusions Butenafine exhibits potent antifungal activity against a wide variety of ocular pathogenic fungi, especially for Aspergillus spp. , Alternaria alternata, Curvularia lunata, and some unusual ocular pathogens and may have a role in future studies of antifungal eye drops and treating fungal keratitis.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2010年第1期38-42,共5页
Chinese Journal of Ophthalmology
基金
基金项目：河南省自然科学基金（031040500）
关键词
苄胺类
萘类
真菌
微生物敏感性试验
抗真菌药
Benzylamines
Naphthalenes
Fungi
Microbial sensitivity tests
Antifungal agents
