摘要
目的 观察体外冲击波（extracorporeal shock wave，ESW）对早中期股骨头坏死（osteonecrosis of the femoral head，ONFH）患者骨髓间质干细胞（mesenchymal stem cells，MSCs）成骨及成脂相关基因表达的影响。方法 取ONFH患者自愿捐赠的骨髓，体外分离并传代培养MSCs。将R代MSCs分为A组（干预组）和B组（对照组）；细胞计数试剂盒检测P3代MSCs不同时间点增殖差异；酶细胞化学和茜素红染色检测如代MSCs碱性磷酸酶（alkaline phosphatase，ALP）含量和P-代MSCs胞外基质矿化程度的差异；RT-PCR检测不同时间点骨钙素（osteocalcin，OC）、核心结合因子αl（core-binding factoralphal，Cbfαl）、过氧化物酶体增殖物激活受体1（peroxisome proliferator-activated receptorγ，PPARγ）表达量的差异。结果A组各时间点（不含第1天）细胞增殖速度均明显强于B组；ALP含量（不含第3天）A组总体水平明显高于B组；茜素红染色示A组矿化结节数为（6．0±1．0）个／视野，B组始终为阴性；Cbfαl在成骨分化的整个过程中均持续表达，A组在各时间点（不含第3天）均明显强于B组；A组OC在各时间点（不含第3、6天）均明显强于B组；PPARy在成骨分化的整个过程中均持续表达，A组在各时间点（不舍第3天）均明显弱于B组．结论适当强度的ESW能够促进MSCs增殖，诱导MSCs向成骨细胞方向分化并抑制MSCs向成脂细胞方向分化是其治疗ONFH的有效机制。
Objective To investigate the effect of extracorporeal shock wave （ESW） on osteogenesisrelated and adipogenic-related genes of mesenchymal stern cells （MSCs） of patients with early or moderate osteonecrosis of the femoral head （ONFH）. Methods MSCs obtained from ONFH patients were divided into Group A and Group B. The proliferation was detected and compared, as well the content of alkaline phosphatase （AI.P） in P30, extracellular matrix mineralization in PT, the expression of core-binding factor alpha 1 （Cbfαl）, osteocalcin （OC） and peroxisome proliferator-activated receptor γ （PPARγ） between two groups at different time points. Results The proliferation of Group A was better than Group B significantly（except the 1st day） ;the ALP content of Group ,4. were higher than that of Group B significantly （except the 3rd day）. The mineralized nodules was （6.0±1.0）/field in Group A, but negative in Group B. Cbfαl sustained expression in the whole process of ESW induced osteoblast differentiation, Group A was significantly stronger than the Group B every day （except the 3rd day）： the OC expression of Group A was significantly earlier and stronger than that of Group B evev day （except the 3rd day and 6th day）. The PPARγ/sustained expression in the whole process of ESW induced osteoblast differentiation, and that in Group A was significant stronger than that in Group B every day（except the 3rd day）. Conclusion The appropriate intensity ESW can promote MSCs proliferation,induce MSCs differentiation into osteoblastic and inhibit MSCs differentiate into fat cells, which may be an effective way of treating ONFH.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2010年第1期84-91,共8页
Chinese Journal of Orthopaedics
关键词
高能量冲击波
股骨头坏死
核心结合因子α1亚基
骨钙素
High-energy sllock waves
Femur head necrosis
Core binding factor alpha i subunit
Osteocalcin
