摘要
目的探讨PBSⅡ+型表达增强激光解析-电离时间飞行质谱仪(surface-enhanced laser desorption/ionization time-of-flight mass spectrometry,SELDI)蛋白芯片技术在系统性红斑狼疮(systemic lupus erythematosus,SLE)诊断、中医证型中的运用。方法选择阴虚内热证轻、中度SLE患者18例作为治疗组和15名健康志愿者作为对照组,均为女性,采用SELDI方法建立治疗组治疗前、中、后及对照组外周血单一核细胞(PBMC)蛋白质指纹图谱,采用ZUCI-PDAS软件包分析处理数据和筛选标志物,以建立诊断模型。结果治疗组退出2例,失访1例,最终完成15例,与对照组比较,SLE组治疗前找出44个蛋白峰差异有统计学意义(P<0.01),10 542 Da m/z和2 554 Da m/z模型的敏感性和特异性均为100%。SLE患者治疗12周与对照组比较,30个峰差异有统计学意义(P<0.01),建立3 365、7 104、3 882和6796Da m/z构成的质谱峰模型敏感性为100%,特异性为93.33%。SLE患者治疗前、中、后样品35个与对照组15个样品比较,55个峰差异有统计学意义(P<0.01),构建模型7 103、3 882、7 143Da m/z的敏感性为100%,特异性为91.43%。结论阴虚内热证SLE治疗前、中、后与对照组比较均存在PBMC蛋白表达模式的显著差异,提示运用SELDI进行SLE诊断的可能,在效应蛋白筛选、活动度评分及中医证候研究等方面的应用值得深入研究。
Objective To investigate the application of surface-enhanced laser desorption ionization timeof-flight mass spectrometry（SELDI） protein chip in diagnosis and Chinese medicie syndrome type researching of systemic lupus erythematosus （SLE）. Methods Eighteen female SLE patients of mild/moderate degree with yin-deficiency caused internal heat syndrome （YDHS） were enrolled in the treatment group, and 15 women healthy volunteers was set up as the control group. Using SELDI method, the pre-, mid- and post-treatment peripheral blood mononuclear cell （PBMC） protein fingerprint of them was created respectively, which was then managed to screen out the markers by using ZUCI-PDAS package for establishing a diagnosis model. Results Study was completed in 15 cases of the treatment group with 2 cases dropped out and 1 case lost. Before treatment, 44 protein peaks in the treatment group were found significantly different to those in the control group （P〈0.01）, and the sensitivity and specificity of the created models, 10 542 Da m/z and 2 554 Da m/z, reached 100%. After a 12-week treatment, 30 peaks were found significantly different between the two groups （P〈0.01）, and the sensitivity of 3 365, 7 104, 3 882 and 6 796 Da m/z created peak models was 100%, its specificity being 93.33 %. Comparing the 35 samples （ pre-, mid- and post-treatment） got from the treatment group with the 15 samples from the control group, significant difference was found in 55 peaks （P 〈0.01 ）, the sensitivity and specificity of the 7 103, 3 882, 7 143 Da m/z created peak models was 100% and 91.43% respectively. Conclusion Significant differences of PBMC protein expression patterns were found between SLE patients of YDHS and healthy persons at times of before, during and after treatment, suggesting that SELDI may be used as a new method to create the diaanosis model, and its application in effecter protein screenina activity scoring and Chinese medicine syndrome type researching are expectable and waiting for further study in depth.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2010年第1期26-29,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.30472218)
关键词
系统性红斑狼疮
表面增强激光解吸离子化飞行时间质谱
阴虚内热证
诊断
systemic lupus erythematosus
surface-enhanced laser desorption/ioniztion time-of-flight mass spectrometry
yin deficiency caused internal heat syndrome
diagnosis
