摘要
采用随机扩增序列区间(ISSR)分子标记技术对蛇莓基株进行鉴定,结果表明6个ISSR引物在22个分株中共扩增出62个条带,每个引物扩增的条带数为4～16条,平均每个引物扩增的条带数为10.3条。采用6种引物对22个克隆分株的DNA扩增共产生28种带谱类型,其中有9种带谱为特异性带谱。综合分析这些带谱,确知这22个克隆分株分属16个基株。由带型可知,通过6个引物中的4种引物就可以把所有的基株鉴定出来,表明ISSR技术在分子水平上鉴定蛇莓的克隆基株是一种行之有效的方法。同时每个引物扩增出来的条带的多态性比例也比较高,平均达到90.3%。
Inter-simple sequence repeat（ISSR）was used to determine the genets of Duchesnea indica.The results show that 6 ISSR primers amplified 62 DNA bands in 22 ramets.Each of the primers amplified 4~16 bands,the average amplified band was 10.3.The use of six primer pairs 22 ramets cloned amplified DNA bands have 28 kinds of type,which has nine specific bands.Comprehensive analysis of these bands,ascertaining that 22 ramets belong to 16 genets.According to the banding patterns,all ramets tested in this study were distinguished from each other by four of 6 selected primers.It was shown that the ISSR technique is a reliable and effective tool to identify the genets of D.indica.At the same time,the polymorphic proportion of the bands amplified by each primer,with an average of 90.3%.
出处
《植物研究》
CAS
CSCD
北大核心
2010年第1期111-115,共5页
Bulletin of Botanical Research
基金
浙江省自然科学基金项目(Y504220)
关键词
蛇莓
克隆鉴定
ISSR
Duchesnea indica
clone identification
ISSR
