摘要
目的:建立参芪颗粒(人参、黄芪、白术、茯苓等)中人参皂苷Rb1、Rc、Rb2与Rd的含量测定方法。方法:采用微管液相色谱梯度洗脱法,色谱柱为MicrosilC18微管色谱柱(150mm×1.0mm,3μm),流动相:乙腈-水梯度洗脱,流速:50μL/min,检测波长为203nm。结果:人参皂苷Rb1在0.24～2.40μg范围内呈良好线性关系(r=0.9999),平均回收率为98.14%,RSD=0.67%(n=6);人参皂苷Rc在0.20～2.00μg范围内呈良好线性关系(r=0.9999),平均回收率为98.04%,RSD=0.99%(n=6);人参皂苷Rb2在0.11～1.10μg范围内呈良好线性关系(r=0.9994),平均回收率为98.79%,RSD=0.75%(n=6);人参皂苷Rd在0.025～0.50μg范围内呈良好线性关系(r=0.9991),平均回收率为97.97%,RSD=1.46%(n=6)。结论:本法简便、准确,能够用于该制剂的质量控制。
AIM： To develop a method for determining ginsenoside Rb1, Rc, Rb2 and Rd in Shenqi Granules （Radix Rhizoma Ginseng, Radix Astragali, Rhizoma Atractylodis macrocephalae, Poria, etc. ） by microbore LC. METHODS： Microbore liquid chromatography was applied. Microsil CIs column（ 150 mm ×1. 0 ram） was used with the mobile phase containing acetonitrile-water for gradient elution. The flow rate was 50μL/min, and the detection wavelength was set at 220 nm. RESULTS ： The relationships between the concentrations and the peak areas of these for components were all linear. The recoveries were 98.14% for ginsenoside Rb1, 98.04% for ginsenoside Rc, 98.79% for ginsenoside Rb2, and 97.97% for ginsenoside Rd respectively. The relative standard deviations （RSD） were 0.67% , 0.99%, 0.75% , and 1.46%, respectively. CONCLUSION： This method is simple, convenient and can be used for quality control.
出处
《中成药》
CAS
CSCD
北大核心
2010年第1期68-71,共4页
Chinese Traditional Patent Medicine
基金
淅江省中医药管理局重点实验室资助项目(2007CB157)
